Assembly and disassembly of DNA polymerase holoenzyme

Daniel J. Sexton, Anthony J. Berdis, Stephen J. Benkovic

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The complex task of genomic replication requires a large collection of proteins properly assembled within the close confines of the replication fork. The mechanism and dynamics of holoenzyme assembly and disassembly have been investigated using steady state and pre-steady state methods as opposed to structural studies, primarily due to the intrinsic transient nature of these protein complexes during DNA replication. The key step in bacteriophage T4 holoenzyme assembly involves ATP hydrolysis, whereas disassembly is mediated by subunit dissociation of the clamp protein in an ATP-independent manner.

Original languageEnglish (US)
Pages (from-to)316-322
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume1
Issue number3
DOIs
StatePublished - Oct 1997

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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