Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Carlos F. Barbas, Anoray S. Kang, Richard A. Lerner, Stephen J. Benkovic

Research output: Contribution to journalArticlepeer-review

972 Scopus citations

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

Original languageEnglish (US)
Pages (from-to)7978-7982
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number18
DOIs
StatePublished - 1991

All Science Journal Classification (ASJC) codes

  • General

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