A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1991|
All Science Journal Classification (ASJC) codes