Assessing a novel room temperature DNA storage medium for forensic biological samples

Steven B. Lee, Kimberly C. Clabaugh, Brie Silva, Kingsley O. Odigie, Michael D. Coble, Odile Loreille, Melissa Scheible, Ron M. Fourney, Jesse Stevens, George R. Carmody, Thomas John Parsons, Arijana Pozder, Arthur J. Eisenberg, Bruce Budowle, Taha Ahmad, Russell W. Miller, Cecelia A. Crouse

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies. A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4× SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA. Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalForensic Science International: Genetics
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2012

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Temperature
DNA
Technology
Agar Gel Electrophoresis
Cell Extracts
Microsatellite Repeats
Coloring Agents
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics

Cite this

Lee, S. B., Clabaugh, K. C., Silva, B., Odigie, K. O., Coble, M. D., Loreille, O., ... Crouse, C. A. (2012). Assessing a novel room temperature DNA storage medium for forensic biological samples. Forensic Science International: Genetics, 6(1), 31-40. https://doi.org/10.1016/j.fsigen.2011.01.008
Lee, Steven B. ; Clabaugh, Kimberly C. ; Silva, Brie ; Odigie, Kingsley O. ; Coble, Michael D. ; Loreille, Odile ; Scheible, Melissa ; Fourney, Ron M. ; Stevens, Jesse ; Carmody, George R. ; Parsons, Thomas John ; Pozder, Arijana ; Eisenberg, Arthur J. ; Budowle, Bruce ; Ahmad, Taha ; Miller, Russell W. ; Crouse, Cecelia A. / Assessing a novel room temperature DNA storage medium for forensic biological samples. In: Forensic Science International: Genetics. 2012 ; Vol. 6, No. 1. pp. 31-40.
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Lee, SB, Clabaugh, KC, Silva, B, Odigie, KO, Coble, MD, Loreille, O, Scheible, M, Fourney, RM, Stevens, J, Carmody, GR, Parsons, TJ, Pozder, A, Eisenberg, AJ, Budowle, B, Ahmad, T, Miller, RW & Crouse, CA 2012, 'Assessing a novel room temperature DNA storage medium for forensic biological samples', Forensic Science International: Genetics, vol. 6, no. 1, pp. 31-40. https://doi.org/10.1016/j.fsigen.2011.01.008

Assessing a novel room temperature DNA storage medium for forensic biological samples. / Lee, Steven B.; Clabaugh, Kimberly C.; Silva, Brie; Odigie, Kingsley O.; Coble, Michael D.; Loreille, Odile; Scheible, Melissa; Fourney, Ron M.; Stevens, Jesse; Carmody, George R.; Parsons, Thomas John; Pozder, Arijana; Eisenberg, Arthur J.; Budowle, Bruce; Ahmad, Taha; Miller, Russell W.; Crouse, Cecelia A.

In: Forensic Science International: Genetics, Vol. 6, No. 1, 01.01.2012, p. 31-40.

Research output: Contribution to journalArticle

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