Assessing effects of alcohol consumption on protein synthesis in striated muscles

Thomas C. Vary, Charles H. Lang

Research output: Chapter in Book/Report/Conference proceedingChapter

30 Scopus citations

Abstract

The development of alcoholic muscle disease, which affects both cardiac and skeletal muscle, leads to increased morbidity and mortality in patients who abuse alcohol. The disease pathology includes myocyte degeneration, loss of striations, and myofilament dissolution, which is consistent with alterations in structural and myofibrillar proteins. One explanation for the changes in myofibrillar architecture is that the expression of cellular proteins may be compromised by ethanol consumption. The dynamic balance of proteins in striated muscle is dependent upon rates of protein synthesis and protein degradation. We have shown that protein synthesis is depressed in striated muscle after either acute alcohol intoxication or chronic alcohol ingestion. The loss of myofibrillar proteins occurs prior to any detection of abnormal muscle function in vivo. It is therefore of major importance to evaluate the regulation of protein turnover after ethanol consumption. This review describes protocols to study protein synthesis either in vivo or under in vitro conditions. The methods can be modified for studies involving transgenic mice allowing mechanisms responsible for the defects in protein synthesis to be dissected.

Original languageEnglish (US)
Title of host publicationAlcohol
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages343-355
Number of pages13
ISBN (Print)9781588299062
DOIs
StatePublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume447
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Vary, T. C., & Lang, C. H. (2008). Assessing effects of alcohol consumption on protein synthesis in striated muscles. In Alcohol: Methods and Protocols (pp. 343-355). (Methods in Molecular Biology; Vol. 447). Humana Press. https://doi.org/10.1007/978-1-59745-242-7_22