Assessing genetic diversity, fine-scale population structure, and demographics in the narrow endemic chittenango ovate amber snail (Novisuccinea chittenangoensis)

T. L. King, M. S. Eackles, A. R. Breisch, R. Niver

Research output: Contribution to journalArticle

Abstract

We document isolation and characterization of 12 tetra-nucleotide microsatellite DNA markers in the hermaphroditic Chittenango ovate amber snail (Novisuccinea chittenangoensis) endemic to Chittenango Falls in central New York State, USA. The markers displayed a moderate level of allelic diversity (averaging 5.2 alleles/locus) and heterozygosity (averaging 58.6%) in the single extant population. Allelic diversity was sufficient to produce unique multilocus genotypes; no indication of selfing was observed among this cosexual species. Minimal deviations from Hardy-Weinberg equilibrium and no linkage disequilibrium were observed when three collections, representing different rock ledge heights, were analyzed separately. A series of analyses identified weak population differentiation among the ledge collections. Demographic analyses suggested each collection has achieved mutation-drift equilibrium. The microsatellite markers developed for N. chittenangoensis yielded sufficient genetic diversity to: (i) distinguish all individuals and assess the level of selfing; (ii) elucidate fine-scale population structuring; and (iii) provide unique demographic perspectives for recovery efforts.

Original languageEnglish (US)
Pages (from-to)439-442
Number of pages4
JournalConservation Genetics Resources
Volume4
Issue number2
DOIs
StatePublished - Dec 1 2012

Fingerprint

Amber
amber
Snails
snail
population structure
demographic statistics
autogamy
Demography
selfing
Microsatellite Repeats
genetic variation
microsatellite repeats
Population
Linkage Disequilibrium
linkage disequilibrium
Genetic Markers
disequilibrium
heterozygosity
mutation
allele

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

@article{44ce036f235340119cb68c193a6842b3,
title = "Assessing genetic diversity, fine-scale population structure, and demographics in the narrow endemic chittenango ovate amber snail (Novisuccinea chittenangoensis)",
abstract = "We document isolation and characterization of 12 tetra-nucleotide microsatellite DNA markers in the hermaphroditic Chittenango ovate amber snail (Novisuccinea chittenangoensis) endemic to Chittenango Falls in central New York State, USA. The markers displayed a moderate level of allelic diversity (averaging 5.2 alleles/locus) and heterozygosity (averaging 58.6{\%}) in the single extant population. Allelic diversity was sufficient to produce unique multilocus genotypes; no indication of selfing was observed among this cosexual species. Minimal deviations from Hardy-Weinberg equilibrium and no linkage disequilibrium were observed when three collections, representing different rock ledge heights, were analyzed separately. A series of analyses identified weak population differentiation among the ledge collections. Demographic analyses suggested each collection has achieved mutation-drift equilibrium. The microsatellite markers developed for N. chittenangoensis yielded sufficient genetic diversity to: (i) distinguish all individuals and assess the level of selfing; (ii) elucidate fine-scale population structuring; and (iii) provide unique demographic perspectives for recovery efforts.",
author = "King, {T. L.} and Eackles, {M. S.} and Breisch, {A. R.} and R. Niver",
year = "2012",
month = "12",
day = "1",
doi = "10.1007/s12686-011-9568-3",
language = "English (US)",
volume = "4",
pages = "439--442",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer Verlag",
number = "2",

}

Assessing genetic diversity, fine-scale population structure, and demographics in the narrow endemic chittenango ovate amber snail (Novisuccinea chittenangoensis). / King, T. L.; Eackles, M. S.; Breisch, A. R.; Niver, R.

In: Conservation Genetics Resources, Vol. 4, No. 2, 01.12.2012, p. 439-442.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Assessing genetic diversity, fine-scale population structure, and demographics in the narrow endemic chittenango ovate amber snail (Novisuccinea chittenangoensis)

AU - King, T. L.

AU - Eackles, M. S.

AU - Breisch, A. R.

AU - Niver, R.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - We document isolation and characterization of 12 tetra-nucleotide microsatellite DNA markers in the hermaphroditic Chittenango ovate amber snail (Novisuccinea chittenangoensis) endemic to Chittenango Falls in central New York State, USA. The markers displayed a moderate level of allelic diversity (averaging 5.2 alleles/locus) and heterozygosity (averaging 58.6%) in the single extant population. Allelic diversity was sufficient to produce unique multilocus genotypes; no indication of selfing was observed among this cosexual species. Minimal deviations from Hardy-Weinberg equilibrium and no linkage disequilibrium were observed when three collections, representing different rock ledge heights, were analyzed separately. A series of analyses identified weak population differentiation among the ledge collections. Demographic analyses suggested each collection has achieved mutation-drift equilibrium. The microsatellite markers developed for N. chittenangoensis yielded sufficient genetic diversity to: (i) distinguish all individuals and assess the level of selfing; (ii) elucidate fine-scale population structuring; and (iii) provide unique demographic perspectives for recovery efforts.

AB - We document isolation and characterization of 12 tetra-nucleotide microsatellite DNA markers in the hermaphroditic Chittenango ovate amber snail (Novisuccinea chittenangoensis) endemic to Chittenango Falls in central New York State, USA. The markers displayed a moderate level of allelic diversity (averaging 5.2 alleles/locus) and heterozygosity (averaging 58.6%) in the single extant population. Allelic diversity was sufficient to produce unique multilocus genotypes; no indication of selfing was observed among this cosexual species. Minimal deviations from Hardy-Weinberg equilibrium and no linkage disequilibrium were observed when three collections, representing different rock ledge heights, were analyzed separately. A series of analyses identified weak population differentiation among the ledge collections. Demographic analyses suggested each collection has achieved mutation-drift equilibrium. The microsatellite markers developed for N. chittenangoensis yielded sufficient genetic diversity to: (i) distinguish all individuals and assess the level of selfing; (ii) elucidate fine-scale population structuring; and (iii) provide unique demographic perspectives for recovery efforts.

UR - http://www.scopus.com/inward/record.url?scp=84892896511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892896511&partnerID=8YFLogxK

U2 - 10.1007/s12686-011-9568-3

DO - 10.1007/s12686-011-9568-3

M3 - Article

AN - SCOPUS:84892896511

VL - 4

SP - 439

EP - 442

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -