Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans

Hadi Nazem-Bokaee, Saratram Gopalakrishnan, James G. Ferry, Thomas K. Wood, Costas D. Maranas

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Methanosarcina acetivorans is a model archaeon with renewed interest due to its unique reversible methane production pathways. However, the mechanism and relevant pathways implicated in (co)utilizing novel carbon substrates in this organism are still not fully understood. This paper provides a comprehensive inventory of thermodynamically feasible routes for anaerobic methane oxidation, co-reactant utilization, and maximum carbon yields of major biofuel candidates by M. acetivorans. Results: Here, an updated genome-scale metabolic model of M. acetivorans is introduced (iMAC868 containing 868 genes, 845 reactions, and 718 metabolites) by integrating information from two previously reconstructed metabolic models (i.e., iVS941 and iMB745), modifying 17 reactions, adding 24 new reactions, and revising 64 gene-protein-reaction associations based on newly available information. The new model establishes improved predictions of growth yields on native substrates and is capable of correctly predicting the knockout outcomes for 27 out of 28 gene deletion mutants. By tracing a bifurcated electron flow mechanism, the iMAC868 model predicts thermodynamically feasible (co)utilization pathway of methane and bicarbonate using various terminal electron acceptors through the reversal of the aceticlastic pathway. Conclusions: This effort paves the way in informing the search for thermodynamically feasible ways of (co)utilizing novel carbon substrates in the domain Archaea.

Original languageEnglish (US)
Article number10
JournalMicrobial Cell Factories
Volume15
Issue number1
DOIs
StatePublished - Jan 17 2016

Fingerprint

Methanosarcina
Carbon Cycle
Biofuels
Methane
Carbon
Archaea
Genes
Electrons
Gene Deletion
Bicarbonates
Substrates
Genome
Equipment and Supplies
Metabolites
Growth
Association reactions
Proteins
Oxidation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans. / Nazem-Bokaee, Hadi; Gopalakrishnan, Saratram; Ferry, James G.; Wood, Thomas K.; Maranas, Costas D.

In: Microbial Cell Factories, Vol. 15, No. 1, 10, 17.01.2016.

Research output: Contribution to journalArticle

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