Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline

Hubert W. Vesper, Laurence Demers, Richard Eastell, Patrick Garnero, Michael Kleerekoper, Simon P. Robins, Apurva K. Srivastava, G. Russell Warnick, Nelson B. Watts, Gary L. Myers

Research output: Contribution to journalReview article

46 Citations (Scopus)

Abstract

Background: Pyridinoline (PYD) and deoxypyridinoline (DPD) are two of the most extensively characterized biochemical bone markers, but the interpretation of results is hampered by biologic and other preanalytical variability. We reviewed factors contributing to preanalytical variation of pyridinium cross-links in urine. Methods: We searched four databases for English-language reports on PYD and/or DPD in urine. Searches were restricted to humans, except for studies of stability, when the search was expanded to other species. The 599 identified articles were supplemented with references from those articles and with articles known to the authors. Results: The mean reported within-day variability was 71% for PYD (range, 57-78%) and 67% for DPD (range, 53-75%). The mean interday variability was 16% for both DPD and PYD (range for PYD, 12-21%; range for DPD, 5-24%). The mean intersubject variabilities across studies were 26% for PYD (range, 12-63%) and 34% for DPD (range, 8-98%) for healthy premenopausal women and 36% (range, 22-61%) and 40%, (range, 27-54%) for postmenopausal women, respectively. Specimen instability and errors in creatinine measurements were additional sources of variability. Conclusions: Intra- and intersubject variability can be reduced by collecting specimens at a specific time of the day and by maintaining similar patient status at each specimen collection regarding factors such as medications and dietary supplements.

Original languageEnglish (US)
Pages (from-to)220-235
Number of pages16
JournalClinical Chemistry
Volume48
Issue number2
StatePublished - Feb 9 2002

Fingerprint

Dietary supplements
Urine
Specimen Handling
Dietary Supplements
pyridinoline
deoxypyridinoline
Creatinine
Bone
Language
Biomarkers
Databases
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Vesper, H. W., Demers, L., Eastell, R., Garnero, P., Kleerekoper, M., Robins, S. P., ... Myers, G. L. (2002). Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline. Clinical Chemistry, 48(2), 220-235.
Vesper, Hubert W. ; Demers, Laurence ; Eastell, Richard ; Garnero, Patrick ; Kleerekoper, Michael ; Robins, Simon P. ; Srivastava, Apurva K. ; Warnick, G. Russell ; Watts, Nelson B. ; Myers, Gary L. / Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline. In: Clinical Chemistry. 2002 ; Vol. 48, No. 2. pp. 220-235.
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abstract = "Background: Pyridinoline (PYD) and deoxypyridinoline (DPD) are two of the most extensively characterized biochemical bone markers, but the interpretation of results is hampered by biologic and other preanalytical variability. We reviewed factors contributing to preanalytical variation of pyridinium cross-links in urine. Methods: We searched four databases for English-language reports on PYD and/or DPD in urine. Searches were restricted to humans, except for studies of stability, when the search was expanded to other species. The 599 identified articles were supplemented with references from those articles and with articles known to the authors. Results: The mean reported within-day variability was 71{\%} for PYD (range, 57-78{\%}) and 67{\%} for DPD (range, 53-75{\%}). The mean interday variability was 16{\%} for both DPD and PYD (range for PYD, 12-21{\%}; range for DPD, 5-24{\%}). The mean intersubject variabilities across studies were 26{\%} for PYD (range, 12-63{\%}) and 34{\%} for DPD (range, 8-98{\%}) for healthy premenopausal women and 36{\%} (range, 22-61{\%}) and 40{\%}, (range, 27-54{\%}) for postmenopausal women, respectively. Specimen instability and errors in creatinine measurements were additional sources of variability. Conclusions: Intra- and intersubject variability can be reduced by collecting specimens at a specific time of the day and by maintaining similar patient status at each specimen collection regarding factors such as medications and dietary supplements.",
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Vesper, HW, Demers, L, Eastell, R, Garnero, P, Kleerekoper, M, Robins, SP, Srivastava, AK, Warnick, GR, Watts, NB & Myers, GL 2002, 'Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline', Clinical Chemistry, vol. 48, no. 2, pp. 220-235.

Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline. / Vesper, Hubert W.; Demers, Laurence; Eastell, Richard; Garnero, Patrick; Kleerekoper, Michael; Robins, Simon P.; Srivastava, Apurva K.; Warnick, G. Russell; Watts, Nelson B.; Myers, Gary L.

In: Clinical Chemistry, Vol. 48, No. 2, 09.02.2002, p. 220-235.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline

AU - Vesper, Hubert W.

AU - Demers, Laurence

AU - Eastell, Richard

AU - Garnero, Patrick

AU - Kleerekoper, Michael

AU - Robins, Simon P.

AU - Srivastava, Apurva K.

AU - Warnick, G. Russell

AU - Watts, Nelson B.

AU - Myers, Gary L.

PY - 2002/2/9

Y1 - 2002/2/9

N2 - Background: Pyridinoline (PYD) and deoxypyridinoline (DPD) are two of the most extensively characterized biochemical bone markers, but the interpretation of results is hampered by biologic and other preanalytical variability. We reviewed factors contributing to preanalytical variation of pyridinium cross-links in urine. Methods: We searched four databases for English-language reports on PYD and/or DPD in urine. Searches were restricted to humans, except for studies of stability, when the search was expanded to other species. The 599 identified articles were supplemented with references from those articles and with articles known to the authors. Results: The mean reported within-day variability was 71% for PYD (range, 57-78%) and 67% for DPD (range, 53-75%). The mean interday variability was 16% for both DPD and PYD (range for PYD, 12-21%; range for DPD, 5-24%). The mean intersubject variabilities across studies were 26% for PYD (range, 12-63%) and 34% for DPD (range, 8-98%) for healthy premenopausal women and 36% (range, 22-61%) and 40%, (range, 27-54%) for postmenopausal women, respectively. Specimen instability and errors in creatinine measurements were additional sources of variability. Conclusions: Intra- and intersubject variability can be reduced by collecting specimens at a specific time of the day and by maintaining similar patient status at each specimen collection regarding factors such as medications and dietary supplements.

AB - Background: Pyridinoline (PYD) and deoxypyridinoline (DPD) are two of the most extensively characterized biochemical bone markers, but the interpretation of results is hampered by biologic and other preanalytical variability. We reviewed factors contributing to preanalytical variation of pyridinium cross-links in urine. Methods: We searched four databases for English-language reports on PYD and/or DPD in urine. Searches were restricted to humans, except for studies of stability, when the search was expanded to other species. The 599 identified articles were supplemented with references from those articles and with articles known to the authors. Results: The mean reported within-day variability was 71% for PYD (range, 57-78%) and 67% for DPD (range, 53-75%). The mean interday variability was 16% for both DPD and PYD (range for PYD, 12-21%; range for DPD, 5-24%). The mean intersubject variabilities across studies were 26% for PYD (range, 12-63%) and 34% for DPD (range, 8-98%) for healthy premenopausal women and 36% (range, 22-61%) and 40%, (range, 27-54%) for postmenopausal women, respectively. Specimen instability and errors in creatinine measurements were additional sources of variability. Conclusions: Intra- and intersubject variability can be reduced by collecting specimens at a specific time of the day and by maintaining similar patient status at each specimen collection regarding factors such as medications and dietary supplements.

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Vesper HW, Demers L, Eastell R, Garnero P, Kleerekoper M, Robins SP et al. Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline. Clinical Chemistry. 2002 Feb 9;48(2):220-235.