Assignment of 44 Ds Insertions to the Linkage Map of Arabidopsis

Fengling Li, Catherine A. Flanagan, Yuju Zhao, Hong Ma, Hai Huang

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations

Abstract

Transposon tagging is a useful tool for biological studies. Transposon insertions have been used to obtain new mutants which are extremely helpful in understanding gene function. These insertions immediately provide a means to isolate the corresponding genes. Transposon tagging has also been used to clone genes previously defined by point mutations. In addition, transposon insertions into cloned genes that lack mutations can be generated to facilitate functional analysis. The maize Ac/Ds transposon elements are known to transpose to local sites with high frequencies and have been shown to function in several dicots. To generate a collection of Ds elements for the purpose of targeted insertional mutagenesis of mapped genes in Arabidopsis, we have mapped 44 Ds insertions by simple sequence length polymorphism (SSLP). Because the Arabidopsis genome project is advancing rapidly, many genes will be discovered whose functions are unknown. The mapped 44 Ds insertions will be a useful resource for post-genome analysis of gene functions in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)109-122
Number of pages14
JournalPlant Molecular Biology Reporter
Volume17
Issue number2
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

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