Although the precise definition for a microtubule-associated protein (MAP) has been the subject of debate, elongation factor-1α (EF-1α) fits the most basic criteria for a MAP [Durso and Cyr, 1994a]. It binds, bundles, stabilizes, and promotes the assembly of microtubules in vitro, and localizes to plant microtubule arrays in situ. In this study, the in vitro and in vivo association of EF-1α with microtubules was further investigated. Analysis of the in vitro binding data for EF-1α and microtubules indicates that EF-1α binds cooperatively to the microtubule lattice. In order to investigate the interaction of EF-1α with microtubules in vivo, GFP fusions to EF-1α or to EF-1α truncates were transiently expressed in living plant cells. Using this method, two putative microtubule-binding domains on EF-1α were identified: one in the N-terminal domain I and one in the C-terminal domain III. The binding of domain I to microtubules in vivo, like the binding of full-length EF-1α, is conditional, and requires incubation in weak, lipophilic organic acids. The binding of domain III to microtubules in vivo, however, is not conditional, and occurs under normal cellular regimes. Furthermore, domain III stabilizes cortical microtubules as determined by their resistance to the anti-microtubule herbicide, oryzalin. Because the accumulation of EF-1α onto microtubules is unconditional in the absence of domain I, we hypothesize that domain I negatively regulates the accumulation of EF-1α onto microtubules in vivo. This hypothesis is discussed in terms of possible regulatory mechanisms that could affect the accumulation of EF-1α onto microtubules within living cells. (C) 2000 Wiley-Liss, Inc.
|Original language||English (US)|
|Number of pages||14|
|Journal||Cell Motility and the Cytoskeleton|
|Publication status||Published - Apr 20 2000|
All Science Journal Classification (ASJC) codes
- Structural Biology
- Cell Biology