Association of bacteria with hydrocephalus in Ugandan infants: Clinical article

Lingling Li, Abinash Padhi, Sylvia L. Ranjeva, Sarah C. Donaldson, Benjamin C. Warf, John Mugamba, Derek Johnson, Zephania Opio, Bhushan M. Jayarao, Vivek Kapur, Mary Poss, Steven Schiff

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Object. Infantile hydrocephalus in East Africa is predominantly postinfectious. The microbial origins remain elusive, since most patients present with postinfectious hydrocephalus after antecedent neonatal sepsis (NS) has resolved. Methods. To characterize this syndrome in Ugandan infants, the authors used polymerase chain reaction targeting bacterial 16S ribosomal DNA from CSF to determine if bacterial residua from recent infections were detectable. Bacteria were identified based on the relationship of genetic sequences obtained with reference bacteria in public databases. The authors evaluated samples from patients presenting during dry and rainy seasons and performed environmental sampling in the villages of patients. Results. Bacterial DNA was recovered from 94% of patients. Gram-negative bacteria in the phylum Proteobacteria were the most commonly detected. Within this phylum, Gammaproteobacteria dominated in patients presenting after infections during the rainy season, and Betaproteobacteria was most common following infections during the dry season. Acinetobacter species were identified in the majority of patients admitted after rainy season infection. Conclusions. Postinfectious hydrocephalus in Ugandan infants appears associated with predominantly enteric gram-negative bacteria. These findings highlight the need for linking these cases with antecedent NS to develop more effective treatment and prevention strategies.

Original languageEnglish (US)
Pages (from-to)73-87
Number of pages15
JournalJournal of Neurosurgery: Pediatrics
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2011

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Hydrocephalus
Bacteria
Infection
Gram-Negative Bacteria
Betaproteobacteria
Gammaproteobacteria
Proteobacteria
Bacterial DNA
Eastern Africa
Acinetobacter
Enterobacteriaceae
Ribosomal DNA
Databases
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Li, Lingling ; Padhi, Abinash ; Ranjeva, Sylvia L. ; Donaldson, Sarah C. ; Warf, Benjamin C. ; Mugamba, John ; Johnson, Derek ; Opio, Zephania ; Jayarao, Bhushan M. ; Kapur, Vivek ; Poss, Mary ; Schiff, Steven. / Association of bacteria with hydrocephalus in Ugandan infants : Clinical article. In: Journal of Neurosurgery: Pediatrics. 2011 ; Vol. 7, No. 1. pp. 73-87.
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Association of bacteria with hydrocephalus in Ugandan infants : Clinical article. / Li, Lingling; Padhi, Abinash; Ranjeva, Sylvia L.; Donaldson, Sarah C.; Warf, Benjamin C.; Mugamba, John; Johnson, Derek; Opio, Zephania; Jayarao, Bhushan M.; Kapur, Vivek; Poss, Mary; Schiff, Steven.

In: Journal of Neurosurgery: Pediatrics, Vol. 7, No. 1, 01.01.2011, p. 73-87.

Research output: Contribution to journalArticle

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AU - Li, Lingling

AU - Padhi, Abinash

AU - Ranjeva, Sylvia L.

AU - Donaldson, Sarah C.

AU - Warf, Benjamin C.

AU - Mugamba, John

AU - Johnson, Derek

AU - Opio, Zephania

AU - Jayarao, Bhushan M.

AU - Kapur, Vivek

AU - Poss, Mary

AU - Schiff, Steven

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N2 - Object. Infantile hydrocephalus in East Africa is predominantly postinfectious. The microbial origins remain elusive, since most patients present with postinfectious hydrocephalus after antecedent neonatal sepsis (NS) has resolved. Methods. To characterize this syndrome in Ugandan infants, the authors used polymerase chain reaction targeting bacterial 16S ribosomal DNA from CSF to determine if bacterial residua from recent infections were detectable. Bacteria were identified based on the relationship of genetic sequences obtained with reference bacteria in public databases. The authors evaluated samples from patients presenting during dry and rainy seasons and performed environmental sampling in the villages of patients. Results. Bacterial DNA was recovered from 94% of patients. Gram-negative bacteria in the phylum Proteobacteria were the most commonly detected. Within this phylum, Gammaproteobacteria dominated in patients presenting after infections during the rainy season, and Betaproteobacteria was most common following infections during the dry season. Acinetobacter species were identified in the majority of patients admitted after rainy season infection. Conclusions. Postinfectious hydrocephalus in Ugandan infants appears associated with predominantly enteric gram-negative bacteria. These findings highlight the need for linking these cases with antecedent NS to develop more effective treatment and prevention strategies.

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