Association of high intracellular, but not serum, heat shock protein 70 with postoperative atrial fibrillation

Kaushik Mandal, Evelyn Torsney, Jan Poloniecki, A. John Camm, Qingbo Xu, Marjan Jahangiri

Research output: Contribution to journalArticle

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Abstract

Background. Atrial fibrillation is a common arrhythmia, after cardiac surgery. Reperfusion injury and inflammation associated with cardiac surgery are thought to be involved in its pathogenesis. We hypothesized that cytoprotective effects associated with heat shock protein 70 (HSP70) could counteract these proarrhythmic insults. We therefore set out to examine the influence of heat shock protein 70 on the incidence of postoperative atrial fibrillation. Methods. We prospectively recruited 80 patients undergoing elective coronary artery bypass surgery. Blood samples were collected preoperatively. Right atrial tissue was obtained at surgery. Incidence of postoperative atrial fibrillation and its duration were noted. Using a nested case-control design, 15 patients who developed atrial fibrillation were matched for operative procedure, age, sex, and β-blocker usage, with 15 controls from the remaining patients. Atrial heat shock protein 70 was subsequently quantified by immunohistochemistry. Serum heat shock protein was measured using enzyme-linked immunosorbent assay and high sensitivity C-reactive protein was determined by immunoturbidometric assay. Results. Intracellular HSP70 level was significantly higher in patients who did not develop atrial fibrillation (35 ± 13 vs 19 ± 15; p = 0.006). Atrial HSP70 level negatively correlated with atrial fibrillation; independent of other risk factors (odds ratio = 0.90; 95% confidence interval 0.84 to 0.99, p = 0.02). Serum HSP70 levels were similar in both groups (p = 0.81) and did not correlate with intracellular levels (p = 0.38). Preoperative C-reactive protein levels were similar in both groups (p = 0.93). Conclusions. Intracellular, but not serum, HSP70 level is negatively correlated with postoperative atrial fibrillation. This suggests a cardioprotective and an antiarrhythmic role for intracellular HSP70.

Original languageEnglish (US)
Pages (from-to)865-871
Number of pages7
JournalAnnals of Thoracic Surgery
Volume79
Issue number3
DOIs
StatePublished - Jan 1 2005

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HSP70 Heat-Shock Proteins
Atrial Fibrillation
Serum
C-Reactive Protein
Thoracic Surgery
Blood Proteins
Incidence
Operative Surgical Procedures
Heat-Shock Proteins
Reperfusion Injury
Coronary Artery Bypass
Cardiac Arrhythmias
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Odds Ratio
Confidence Intervals
Inflammation

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Mandal, Kaushik ; Torsney, Evelyn ; Poloniecki, Jan ; Camm, A. John ; Xu, Qingbo ; Jahangiri, Marjan. / Association of high intracellular, but not serum, heat shock protein 70 with postoperative atrial fibrillation. In: Annals of Thoracic Surgery. 2005 ; Vol. 79, No. 3. pp. 865-871.
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abstract = "Background. Atrial fibrillation is a common arrhythmia, after cardiac surgery. Reperfusion injury and inflammation associated with cardiac surgery are thought to be involved in its pathogenesis. We hypothesized that cytoprotective effects associated with heat shock protein 70 (HSP70) could counteract these proarrhythmic insults. We therefore set out to examine the influence of heat shock protein 70 on the incidence of postoperative atrial fibrillation. Methods. We prospectively recruited 80 patients undergoing elective coronary artery bypass surgery. Blood samples were collected preoperatively. Right atrial tissue was obtained at surgery. Incidence of postoperative atrial fibrillation and its duration were noted. Using a nested case-control design, 15 patients who developed atrial fibrillation were matched for operative procedure, age, sex, and β-blocker usage, with 15 controls from the remaining patients. Atrial heat shock protein 70 was subsequently quantified by immunohistochemistry. Serum heat shock protein was measured using enzyme-linked immunosorbent assay and high sensitivity C-reactive protein was determined by immunoturbidometric assay. Results. Intracellular HSP70 level was significantly higher in patients who did not develop atrial fibrillation (35 ± 13 vs 19 ± 15; p = 0.006). Atrial HSP70 level negatively correlated with atrial fibrillation; independent of other risk factors (odds ratio = 0.90; 95{\%} confidence interval 0.84 to 0.99, p = 0.02). Serum HSP70 levels were similar in both groups (p = 0.81) and did not correlate with intracellular levels (p = 0.38). Preoperative C-reactive protein levels were similar in both groups (p = 0.93). Conclusions. Intracellular, but not serum, HSP70 level is negatively correlated with postoperative atrial fibrillation. This suggests a cardioprotective and an antiarrhythmic role for intracellular HSP70.",
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Association of high intracellular, but not serum, heat shock protein 70 with postoperative atrial fibrillation. / Mandal, Kaushik; Torsney, Evelyn; Poloniecki, Jan; Camm, A. John; Xu, Qingbo; Jahangiri, Marjan.

In: Annals of Thoracic Surgery, Vol. 79, No. 3, 01.01.2005, p. 865-871.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of high intracellular, but not serum, heat shock protein 70 with postoperative atrial fibrillation

AU - Mandal, Kaushik

AU - Torsney, Evelyn

AU - Poloniecki, Jan

AU - Camm, A. John

AU - Xu, Qingbo

AU - Jahangiri, Marjan

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Background. Atrial fibrillation is a common arrhythmia, after cardiac surgery. Reperfusion injury and inflammation associated with cardiac surgery are thought to be involved in its pathogenesis. We hypothesized that cytoprotective effects associated with heat shock protein 70 (HSP70) could counteract these proarrhythmic insults. We therefore set out to examine the influence of heat shock protein 70 on the incidence of postoperative atrial fibrillation. Methods. We prospectively recruited 80 patients undergoing elective coronary artery bypass surgery. Blood samples were collected preoperatively. Right atrial tissue was obtained at surgery. Incidence of postoperative atrial fibrillation and its duration were noted. Using a nested case-control design, 15 patients who developed atrial fibrillation were matched for operative procedure, age, sex, and β-blocker usage, with 15 controls from the remaining patients. Atrial heat shock protein 70 was subsequently quantified by immunohistochemistry. Serum heat shock protein was measured using enzyme-linked immunosorbent assay and high sensitivity C-reactive protein was determined by immunoturbidometric assay. Results. Intracellular HSP70 level was significantly higher in patients who did not develop atrial fibrillation (35 ± 13 vs 19 ± 15; p = 0.006). Atrial HSP70 level negatively correlated with atrial fibrillation; independent of other risk factors (odds ratio = 0.90; 95% confidence interval 0.84 to 0.99, p = 0.02). Serum HSP70 levels were similar in both groups (p = 0.81) and did not correlate with intracellular levels (p = 0.38). Preoperative C-reactive protein levels were similar in both groups (p = 0.93). Conclusions. Intracellular, but not serum, HSP70 level is negatively correlated with postoperative atrial fibrillation. This suggests a cardioprotective and an antiarrhythmic role for intracellular HSP70.

AB - Background. Atrial fibrillation is a common arrhythmia, after cardiac surgery. Reperfusion injury and inflammation associated with cardiac surgery are thought to be involved in its pathogenesis. We hypothesized that cytoprotective effects associated with heat shock protein 70 (HSP70) could counteract these proarrhythmic insults. We therefore set out to examine the influence of heat shock protein 70 on the incidence of postoperative atrial fibrillation. Methods. We prospectively recruited 80 patients undergoing elective coronary artery bypass surgery. Blood samples were collected preoperatively. Right atrial tissue was obtained at surgery. Incidence of postoperative atrial fibrillation and its duration were noted. Using a nested case-control design, 15 patients who developed atrial fibrillation were matched for operative procedure, age, sex, and β-blocker usage, with 15 controls from the remaining patients. Atrial heat shock protein 70 was subsequently quantified by immunohistochemistry. Serum heat shock protein was measured using enzyme-linked immunosorbent assay and high sensitivity C-reactive protein was determined by immunoturbidometric assay. Results. Intracellular HSP70 level was significantly higher in patients who did not develop atrial fibrillation (35 ± 13 vs 19 ± 15; p = 0.006). Atrial HSP70 level negatively correlated with atrial fibrillation; independent of other risk factors (odds ratio = 0.90; 95% confidence interval 0.84 to 0.99, p = 0.02). Serum HSP70 levels were similar in both groups (p = 0.81) and did not correlate with intracellular levels (p = 0.38). Preoperative C-reactive protein levels were similar in both groups (p = 0.93). Conclusions. Intracellular, but not serum, HSP70 level is negatively correlated with postoperative atrial fibrillation. This suggests a cardioprotective and an antiarrhythmic role for intracellular HSP70.

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U2 - 10.1016/j.athoracsur.2004.08.018

DO - 10.1016/j.athoracsur.2004.08.018

M3 - Article

VL - 79

SP - 865

EP - 871

JO - Annals of Thoracic Surgery

JF - Annals of Thoracic Surgery

SN - 0003-4975

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