Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina

Salvatore Stella, Alejandro Vila, Albert Y. Hung, Michael E. Rome, Uyenchi Huynh, Morgan Sheng, Hans Juergen Kreienkamp, Nicholas C. Brecha

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Photoreceptor terminals contain post-synaptic density (PSD) proteins e.g., PSD-95/PSD-93, but their role at photoreceptor synapses is not known. PSDs are generally restricted to post-synaptic boutons in central neurons and form scaffolding with multiple proteins that have structural and functional roles in neuronal signaling. The Shank family of proteins (Shank 1-3) functions as putative anchoring proteins for PSDs and is involved in the organization of cytoskeletal/signaling complexes in neurons. Specifically, Shank 1 is restricted to neurons and interacts with both receptors and signaling molecules at central neurons to regulate plasticity. However, it is not known whether Shank 1 is expressed at photoreceptor terminals. In this study we have investigated Shank 1A localization in the outer retina at photoreceptor terminals. We find that Shank 1A is expressed presynaptically in cone pedicles, but not rod spherules, and it is absent from mice in which the Shank 1 gene is deleted. Shank 1A co-localizes with PSD-95, peanut agglutinin, a marker of cone terminals, and glycogen phosphorylase, a cone specific marker. These findings provide convincing evidence for Shank 1A expression in both the inner and outer plexiform layers, and indicate a potential role for PSD-95/Shank 1 complexes at cone synapses in the outer retina.

Original languageEnglish (US)
Article numbere43463
JournalPloS one
Volume7
Issue number9
DOIs
StatePublished - Sep 12 2012

Fingerprint

scaffolding proteins
Retinal Cone Photoreceptor Cells
Post-Synaptic Density
cones (retina)
retina
Neurons
Retina
Cones
photoreceptors
neurons
synapse
Proteins
Synapses
Peanut Agglutinin
Glycogen Phosphorylase
rods (retina)
phosphorylase
proteins
structural proteins
Plasticity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Stella, Salvatore ; Vila, Alejandro ; Hung, Albert Y. ; Rome, Michael E. ; Huynh, Uyenchi ; Sheng, Morgan ; Kreienkamp, Hans Juergen ; Brecha, Nicholas C. / Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina. In: PloS one. 2012 ; Vol. 7, No. 9.
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abstract = "Photoreceptor terminals contain post-synaptic density (PSD) proteins e.g., PSD-95/PSD-93, but their role at photoreceptor synapses is not known. PSDs are generally restricted to post-synaptic boutons in central neurons and form scaffolding with multiple proteins that have structural and functional roles in neuronal signaling. The Shank family of proteins (Shank 1-3) functions as putative anchoring proteins for PSDs and is involved in the organization of cytoskeletal/signaling complexes in neurons. Specifically, Shank 1 is restricted to neurons and interacts with both receptors and signaling molecules at central neurons to regulate plasticity. However, it is not known whether Shank 1 is expressed at photoreceptor terminals. In this study we have investigated Shank 1A localization in the outer retina at photoreceptor terminals. We find that Shank 1A is expressed presynaptically in cone pedicles, but not rod spherules, and it is absent from mice in which the Shank 1 gene is deleted. Shank 1A co-localizes with PSD-95, peanut agglutinin, a marker of cone terminals, and glycogen phosphorylase, a cone specific marker. These findings provide convincing evidence for Shank 1A expression in both the inner and outer plexiform layers, and indicate a potential role for PSD-95/Shank 1 complexes at cone synapses in the outer retina.",
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Stella, S, Vila, A, Hung, AY, Rome, ME, Huynh, U, Sheng, M, Kreienkamp, HJ & Brecha, NC 2012, 'Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina', PloS one, vol. 7, no. 9, e43463. https://doi.org/10.1371/journal.pone.0043463

Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina. / Stella, Salvatore; Vila, Alejandro; Hung, Albert Y.; Rome, Michael E.; Huynh, Uyenchi; Sheng, Morgan; Kreienkamp, Hans Juergen; Brecha, Nicholas C.

In: PloS one, Vol. 7, No. 9, e43463, 12.09.2012.

Research output: Contribution to journalArticle

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AU - Stella, Salvatore

AU - Vila, Alejandro

AU - Hung, Albert Y.

AU - Rome, Michael E.

AU - Huynh, Uyenchi

AU - Sheng, Morgan

AU - Kreienkamp, Hans Juergen

AU - Brecha, Nicholas C.

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