Association of soil chemistry and other factors with spatially distributed Burkholderia mallei DNA in Punjab province, Pakistan

Muhammad Asad Ali, Khushi Muhammad, Aftab Ahmad Anjum, Mansur Ud Din Ahmad, Masood Rabbani, Muhammad Zubair Shabbir, Arfan Ahmad, Muhammad Nawaz, Muhammad Tasleem Ghori, Javed Muhammad, Haroon Rashid Chaudhry, Tariq Jamil, Muhammad Haisem, Tasmia Awan, Rais Ahmad, Bhushan M. Jayarao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Association of soil chemistry and other risk factors with soil borne Burkholderia mallei (B. mallei) DNA in eight districts (Sheikhupura, Gujranwala, Faisalabad, Sargodha, Chakwal, Attock, Sahiwal, Dera Ghazi Khan) of Punjab province, Pakistan, was studied. A total of 22 soil samples (n=11, each from soil positive and negative for B. mallei DNA) were processed for chemical analysis from B. mallei positive districts (Sheikhupura and Chakwal). The relationship between soil composition, absence or presence of the pathogen was ascertained. In soil samples of Chakwal district, DNA of B. mallei was found to be highly associated with 0.80 to 39.20% range of moisture contents (p=0.008) and 1.74 to 21.75 mg/Kg of P (p=0.050). The association in Sheikhupura district was with sodium (1.90 to 133.59 mg/Kg; p=0.018) and moisture (0.80 to 39.20%; p=0.026). The odds of detecting DNA of B. mallei was 1.4, 6.8, 5.0, 2.8 and 10.6 higher when soil sample sites were < 500 meters from vehicular traffic roads, < 01 km from animal markets, < 100 meters from canal, < 1000 animals and < 300 houses/village, respectively. While the odds of detecting DNA of B. mallei were 0.1, 0.3, 0.4, 0.2 and 0.5 when soil sample sites were > 500 meters from vehicular traffic roads, > 01 km from animal markets, > 100 meters from canal, > 1000 animals and > 300 houses/village. Soilborne B. mallei DNA is more likely to be detected in areas closer to roads having vehicular traffic along with interstate routes and soil containing low levels of moisture.

Original languageEnglish (US)
Title of host publicationProceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
EditorsMuhammad Zafar-uz-Zaman
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages220-236
Number of pages17
ISBN (Electronic)9781467390736
DOIs
StatePublished - Mar 1 2017
Event14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 - Islamabad, Pakistan
Duration: Jan 10 2017Jan 14 2017

Publication series

NameProceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

Other

Other14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
CountryPakistan
CityIslamabad
Period1/10/171/14/17

All Science Journal Classification (ASJC) codes

  • Computer Science (miscellaneous)

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    Ali, M. A., Muhammad, K., Anjum, A. A., Ahmad, M. U. D., Rabbani, M., Shabbir, M. Z., Ahmad, A., Nawaz, M., Ghori, M. T., Muhammad, J., Chaudhry, H. R., Jamil, T., Haisem, M., Awan, T., Ahmad, R., & Jayarao, B. M. (2017). Association of soil chemistry and other factors with spatially distributed Burkholderia mallei DNA in Punjab province, Pakistan. In M. Zafar-uz-Zaman (Ed.), Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 (pp. 220-236). [7868058] (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IBCAST.2017.7868058