Abstract

The anti-inflammatory genes, haem oxygenase 1 (HO-1, HMOX1) rs2071746 (unrestricted model: p = 9.07 × 10 -4 ; recessive model: p = 4.99 × 10 -4 ; multiplicative model: p = 0.0009; and additive model: p = 1.87 × 10 -4 ) and interleukin-10 (IL-10) rs1800872 (dominant model: p = 0.0277) have been associated with paediatric inflammatory bowel disease. The present family-based case-trio study (n = 52) examined HO-1 gene expression in the presence of proinflammatory lipopolysaccharide and tumour necrosis factor-alpha in four B lymphocyte cell lines established from children with inflammatory bowel disease and demonstrated that mutations in IL-10 and IL-10 receptor B reduced HO-1 messenger RNA expression. This observation supports our hypothesis that HO-1 is regulated by the IL-10/ STAT3 pathway and that both genes (IL10 and STAT3) could be involved in the pathogenesis of inflammatory bowel disease. We also compared HO-1 expression in diseased intestinal tissues with adjacent normal tissues from adults with inflammatory bowel disease. Of the 17 Crohn’s disease patients, HO-1 expression in diseased tissues was downregulated in 9 patients (53%) and of the 10 ulcerative colitis patients HO-1 was downregulated in 7 patients (70%), compared with adjacent normal tissues. The downregulation of HO-1 gene expression may lower anti-inflammatory effects and worsen tissue injury in affected areas by inflammatory bowel disease.

Original languageEnglish (US)
Article numberw14456
JournalSwiss Medical Weekly
Volume147
DOIs
StatePublished - Aug 2 2017

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Heme Oxygenase (Decyclizing)
Inflammatory Bowel Diseases
Interleukin-10
Down-Regulation
Genes
Anti-Inflammatory Agents
Interleukin-10 Receptors
Gene Expression
Intestinal Diseases
Ulcerative Colitis
Crohn Disease
Lipopolysaccharides
B-Lymphocytes
Tumor Necrosis Factor-alpha
Pediatrics
Cell Line
Messenger RNA
Mutation
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lin, Zhenwu ; Hao, Han ; Hegarty, John P. ; Lin, Tony R. ; Wang, Yunhua ; Harris, Leonard R. ; Xu, He N. ; Wu, Rongling ; Thomas, Neal J. ; Floros, Joanna. / Association of the haem oxygenase-1 gene with inflammatory bowel disease. In: Swiss Medical Weekly. 2017 ; Vol. 147.
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abstract = "The anti-inflammatory genes, haem oxygenase 1 (HO-1, HMOX1) rs2071746 (unrestricted model: p = 9.07 × 10 -4 ; recessive model: p = 4.99 × 10 -4 ; multiplicative model: p = 0.0009; and additive model: p = 1.87 × 10 -4 ) and interleukin-10 (IL-10) rs1800872 (dominant model: p = 0.0277) have been associated with paediatric inflammatory bowel disease. The present family-based case-trio study (n = 52) examined HO-1 gene expression in the presence of proinflammatory lipopolysaccharide and tumour necrosis factor-alpha in four B lymphocyte cell lines established from children with inflammatory bowel disease and demonstrated that mutations in IL-10 and IL-10 receptor B reduced HO-1 messenger RNA expression. This observation supports our hypothesis that HO-1 is regulated by the IL-10/ STAT3 pathway and that both genes (IL10 and STAT3) could be involved in the pathogenesis of inflammatory bowel disease. We also compared HO-1 expression in diseased intestinal tissues with adjacent normal tissues from adults with inflammatory bowel disease. Of the 17 Crohn’s disease patients, HO-1 expression in diseased tissues was downregulated in 9 patients (53{\%}) and of the 10 ulcerative colitis patients HO-1 was downregulated in 7 patients (70{\%}), compared with adjacent normal tissues. The downregulation of HO-1 gene expression may lower anti-inflammatory effects and worsen tissue injury in affected areas by inflammatory bowel disease.",
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Association of the haem oxygenase-1 gene with inflammatory bowel disease. / Lin, Zhenwu; Hao, Han; Hegarty, John P.; Lin, Tony R.; Wang, Yunhua; Harris, Leonard R.; Xu, He N.; Wu, Rongling; Thomas, Neal J.; Floros, Joanna.

In: Swiss Medical Weekly, Vol. 147, w14456, 02.08.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of the haem oxygenase-1 gene with inflammatory bowel disease

AU - Lin, Zhenwu

AU - Hao, Han

AU - Hegarty, John P.

AU - Lin, Tony R.

AU - Wang, Yunhua

AU - Harris, Leonard R.

AU - Xu, He N.

AU - Wu, Rongling

AU - Thomas, Neal J.

AU - Floros, Joanna

PY - 2017/8/2

Y1 - 2017/8/2

N2 - The anti-inflammatory genes, haem oxygenase 1 (HO-1, HMOX1) rs2071746 (unrestricted model: p = 9.07 × 10 -4 ; recessive model: p = 4.99 × 10 -4 ; multiplicative model: p = 0.0009; and additive model: p = 1.87 × 10 -4 ) and interleukin-10 (IL-10) rs1800872 (dominant model: p = 0.0277) have been associated with paediatric inflammatory bowel disease. The present family-based case-trio study (n = 52) examined HO-1 gene expression in the presence of proinflammatory lipopolysaccharide and tumour necrosis factor-alpha in four B lymphocyte cell lines established from children with inflammatory bowel disease and demonstrated that mutations in IL-10 and IL-10 receptor B reduced HO-1 messenger RNA expression. This observation supports our hypothesis that HO-1 is regulated by the IL-10/ STAT3 pathway and that both genes (IL10 and STAT3) could be involved in the pathogenesis of inflammatory bowel disease. We also compared HO-1 expression in diseased intestinal tissues with adjacent normal tissues from adults with inflammatory bowel disease. Of the 17 Crohn’s disease patients, HO-1 expression in diseased tissues was downregulated in 9 patients (53%) and of the 10 ulcerative colitis patients HO-1 was downregulated in 7 patients (70%), compared with adjacent normal tissues. The downregulation of HO-1 gene expression may lower anti-inflammatory effects and worsen tissue injury in affected areas by inflammatory bowel disease.

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Lin Z, Hao H, Hegarty JP, Lin TR, Wang Y, Harris LR et al. Association of the haem oxygenase-1 gene with inflammatory bowel disease. Swiss Medical Weekly. 2017 Aug 2;147. w14456. https://doi.org/10.4414/smw.2017.14456