ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole

Bhanupriya Madarampalli, Yunsheng Yuan, Dan Liu, Kathleen Lengel, Yidi Xu, Guangfu Li, Jinming Yang, Xinyuan Liu, Zhimin Lu, David X. Liu

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Although it is known that the centrioles play instructive roles in pericentriolar material (PCM) assembly and that the PCM is essential for proper centriole formation, the mechanism that governs centriole-PCM interaction is poorly understood. Here, we show that ATF5 forms a characteristic 9-fold symmetrical ring structure in the inner layer of the PCM outfitting the proximal end of the mother centriole. ATF5 controls the centriole-PCM interaction in a cell-cycle- and centriole-age-dependent manner. Interaction of ATF5 with polyglutamylated tubulin (PGT) on the mother centriole and with PCNT in the PCM renders ATF5 as a required molecule in mother centriole-directed PCM accumulation and in PCM-dependent centriole formation. ATF5 depletion blocks PCM accumulation at the centrosome and causes fragmentation of centrioles, leading to the formation of multi-polar mitotic spindles and genomic instability. These data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the PCM.

Original languageEnglish (US)
Pages (from-to)580-592
Number of pages13
JournalCell
Volume162
Issue number3
DOIs
StatePublished - Aug 1 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Madarampalli, B., Yuan, Y., Liu, D., Lengel, K., Xu, Y., Li, G., Yang, J., Liu, X., Lu, Z., & Liu, D. X. (2015). ATF5 Connects the Pericentriolar Materials to the Proximal End of the Mother Centriole. Cell, 162(3), 580-592. https://doi.org/10.1016/j.cell.2015.06.055