ATP hydrolyzing salivary enzymes of caterpillars suppress plant defenses

Shuang Wu, Michelle Peiffer, Dawn S. Luthe, Gary Felton

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The oral secretions of herbivores are important recognition cues that can be used by plants to mediate induced defenses. In this study, a degradation of adenosine-5′-triphosphate (ATP) in tomato leaves was detected after treatment with Helicoverpa zea saliva. Correspondingly, a high level of ATPase activity in saliva was detected and three ATP hydrolyzing enzymes: apyrase, ATP synthase and ATPase 13A1 were identified in salivary glands. To determine the functions of these proteins in mediating defenses, they were cloned from H. zea and expressed in Escherichia coli. By applying the purified expressed apyrase, ATP synthase or ATPase 13A1 to wounded tomato leaves, it was determined that these ATP hydrolyzing enzymes suppressed the defensive genes regulated by the jasmonic acid and ethylene pathways in tomato plant. Suppression of glandular trichome production was also observed after treatment. Blood-feeding arthropods employ 5′-nucleotidase family of apyrases to circumvent host responses and the H. zea apyrase, is also a member of this family. The comparatively high degree of sequence similarity of the H. zea salivary apyrase with mosquito apyrases suggests a broader evolutionary role for salivary apyrases than previously envisioned.

Original languageEnglish (US)
Article numbere41947
JournalPloS one
Volume7
Issue number7
DOIs
StatePublished - Jul 25 2012

Fingerprint

apyrase
Apyrase
adenosine triphosphate
Adenosine
insect larvae
Adenosine Triphosphate
Helicoverpa zea
Enzymes
Zea mays
enzymes
Lycopersicon esculentum
adenosinetriphosphatase
Adenosine Triphosphatases
tomatoes
saliva
Saliva
5'-nucleotidase
Trichomes
5'-Nucleotidase
Herbivory

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wu, Shuang ; Peiffer, Michelle ; Luthe, Dawn S. ; Felton, Gary. / ATP hydrolyzing salivary enzymes of caterpillars suppress plant defenses. In: PloS one. 2012 ; Vol. 7, No. 7.
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ATP hydrolyzing salivary enzymes of caterpillars suppress plant defenses. / Wu, Shuang; Peiffer, Michelle; Luthe, Dawn S.; Felton, Gary.

In: PloS one, Vol. 7, No. 7, e41947, 25.07.2012.

Research output: Contribution to journalArticle

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