Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA-Wrapped Single-Walled Carbon Nanotubes

Massooma Pirbhai, Swetha Chandrasekar, Ming Zheng, Tetyana Ignatova, Vyacheslav Rotkin, Sabrina S. Jedlicka

Research output: Contribution to journalArticle

Abstract

Nanostructured biomaterials are extensively explored in clinical imaging and in gene/drug delivery applications. However, limited studies are performed that examine the influence that nanomaterials may have on cell behavior over long time scales at nonlethal concentrations. This study is designed to investigate whether carbon nanotubes are able to augment cell behavior at low concentrations. Single-walled carbon nanotubes are introduced to neural stem cells at different stages of differentiation at concentrations as low as 5 ng mL −1 . Results demonstrate that in this particular cell model, nanotube uptake is mediated by endocytosis. Differentiation is augmented, especially when nanotubes are introduced to cells in an actively dividing state. Significant increases in neuronal cell population are observed over the control specimens. While the mechanisms behind this observation are yet unknown, this study demonstrates that low concentrations of internalized nanomaterials can significantly alter the differentiation profile of a stem cell line.

Original languageEnglish (US)
Article number1800321
JournalAdvanced Biosystems
Volume3
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Carbon Nanotubes
Neural Stem Cells
Single-walled carbon nanotubes (SWCN)
Stem cells
Nanostructured materials
Nanotubes
Cell Differentiation
Medical imaging
Biocompatible Materials
Drug delivery
Biomaterials
Nanostructures
Carbon nanotubes
Genes
Cells
Endocytosis
Stem Cells
Cell Line
Pharmaceutical Preparations
Population

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biomaterials
  • Biomedical Engineering

Cite this

Pirbhai, Massooma ; Chandrasekar, Swetha ; Zheng, Ming ; Ignatova, Tetyana ; Rotkin, Vyacheslav ; Jedlicka, Sabrina S. / Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA-Wrapped Single-Walled Carbon Nanotubes. In: Advanced Biosystems. 2019 ; Vol. 3, No. 4.
@article{dc5bdbcea9e44a20a148c612e28f1ccc,
title = "Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA-Wrapped Single-Walled Carbon Nanotubes",
abstract = "Nanostructured biomaterials are extensively explored in clinical imaging and in gene/drug delivery applications. However, limited studies are performed that examine the influence that nanomaterials may have on cell behavior over long time scales at nonlethal concentrations. This study is designed to investigate whether carbon nanotubes are able to augment cell behavior at low concentrations. Single-walled carbon nanotubes are introduced to neural stem cells at different stages of differentiation at concentrations as low as 5 ng mL −1 . Results demonstrate that in this particular cell model, nanotube uptake is mediated by endocytosis. Differentiation is augmented, especially when nanotubes are introduced to cells in an actively dividing state. Significant increases in neuronal cell population are observed over the control specimens. While the mechanisms behind this observation are yet unknown, this study demonstrates that low concentrations of internalized nanomaterials can significantly alter the differentiation profile of a stem cell line.",
author = "Massooma Pirbhai and Swetha Chandrasekar and Ming Zheng and Tetyana Ignatova and Vyacheslav Rotkin and Jedlicka, {Sabrina S.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1002/adbi.201800321",
language = "English (US)",
volume = "3",
journal = "Advanced Biosystems",
issn = "2366-7478",
publisher = "Wiley-VCH Verlag",
number = "4",

}

Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA-Wrapped Single-Walled Carbon Nanotubes. / Pirbhai, Massooma; Chandrasekar, Swetha; Zheng, Ming; Ignatova, Tetyana; Rotkin, Vyacheslav; Jedlicka, Sabrina S.

In: Advanced Biosystems, Vol. 3, No. 4, 1800321, 01.04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Augmentation of C17.2 Neural Stem Cell Differentiation via Uptake of Low Concentrations of ssDNA-Wrapped Single-Walled Carbon Nanotubes

AU - Pirbhai, Massooma

AU - Chandrasekar, Swetha

AU - Zheng, Ming

AU - Ignatova, Tetyana

AU - Rotkin, Vyacheslav

AU - Jedlicka, Sabrina S.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Nanostructured biomaterials are extensively explored in clinical imaging and in gene/drug delivery applications. However, limited studies are performed that examine the influence that nanomaterials may have on cell behavior over long time scales at nonlethal concentrations. This study is designed to investigate whether carbon nanotubes are able to augment cell behavior at low concentrations. Single-walled carbon nanotubes are introduced to neural stem cells at different stages of differentiation at concentrations as low as 5 ng mL −1 . Results demonstrate that in this particular cell model, nanotube uptake is mediated by endocytosis. Differentiation is augmented, especially when nanotubes are introduced to cells in an actively dividing state. Significant increases in neuronal cell population are observed over the control specimens. While the mechanisms behind this observation are yet unknown, this study demonstrates that low concentrations of internalized nanomaterials can significantly alter the differentiation profile of a stem cell line.

AB - Nanostructured biomaterials are extensively explored in clinical imaging and in gene/drug delivery applications. However, limited studies are performed that examine the influence that nanomaterials may have on cell behavior over long time scales at nonlethal concentrations. This study is designed to investigate whether carbon nanotubes are able to augment cell behavior at low concentrations. Single-walled carbon nanotubes are introduced to neural stem cells at different stages of differentiation at concentrations as low as 5 ng mL −1 . Results demonstrate that in this particular cell model, nanotube uptake is mediated by endocytosis. Differentiation is augmented, especially when nanotubes are introduced to cells in an actively dividing state. Significant increases in neuronal cell population are observed over the control specimens. While the mechanisms behind this observation are yet unknown, this study demonstrates that low concentrations of internalized nanomaterials can significantly alter the differentiation profile of a stem cell line.

UR - http://www.scopus.com/inward/record.url?scp=85062550776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062550776&partnerID=8YFLogxK

U2 - 10.1002/adbi.201800321

DO - 10.1002/adbi.201800321

M3 - Article

AN - SCOPUS:85062550776

VL - 3

JO - Advanced Biosystems

JF - Advanced Biosystems

SN - 2366-7478

IS - 4

M1 - 1800321

ER -