AurF from Streptomyces thioluteus and a possible new family of manganese/iron oxygenases

Carsten Krebs, Megan L. Matthews, Wei Jiang, Joseph M. Bollinger, Jr.

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We recently reported that the R2 subunit of class Ic ribonucleotide reductase from Chlamydia trachomatis contains a heterodinuclear Mn/Fe redox cofactor [Jiang, W., Yun, D., Saleh, L., Barr, E. W., Xing, G., Hoffart, L. M., Maslak, M.-A., Krebs, C., and Bollinger, J. M., Jr. (2007) Science 316, 1188-1191]. The N-oxygenase, AurF, from Streptomyces thioluteus catalyzes the six-electron oxidation of p-aminobenzoate to p-nitrobenzoate and contains the EX2HX60-180EX2H sequence motif previously used to identify proteins with non-heme diiron clusters. Two research groups independently obtained evidence for the presence of iron and manganese in preparations of AurF. The electron paramagnetic resonance (EPR) spectrum of purified, resting AurF presented in one of these studies is markedly similar to the spectrum of the MnIII/FeIII form of C. trachomatis R2. We propose that S. thioluteus AurF also may harbor a heterodinuclear Mn/Fe cofactor, which it may use to activate O2 for oxidation of the aryl amine to the nitro compound. Hypothetical proteins encoded in the genomes of several other bacteria have similar sequences and may also be members of this nascent family of oxygen-activating Mn/Fe proteins.

Original languageEnglish (US)
Pages (from-to)10413-10418
Number of pages6
JournalBiochemistry
Volume46
Issue number37
DOIs
StatePublished - Sep 18 2007

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Oxygenases
Streptomyces
Manganese
Iron
Chlamydia trachomatis
para-Aminobenzoates
Nitro Compounds
Nitrobenzoates
Ribonucleotide Reductases
Oxidation
Proteins
Electron Spin Resonance Spectroscopy
Ports and harbors
Oxidation-Reduction
Amines
Paramagnetic resonance
Bacteria
Genes
Genome
Electrons

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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title = "AurF from Streptomyces thioluteus and a possible new family of manganese/iron oxygenases",
abstract = "We recently reported that the R2 subunit of class Ic ribonucleotide reductase from Chlamydia trachomatis contains a heterodinuclear Mn/Fe redox cofactor [Jiang, W., Yun, D., Saleh, L., Barr, E. W., Xing, G., Hoffart, L. M., Maslak, M.-A., Krebs, C., and Bollinger, J. M., Jr. (2007) Science 316, 1188-1191]. The N-oxygenase, AurF, from Streptomyces thioluteus catalyzes the six-electron oxidation of p-aminobenzoate to p-nitrobenzoate and contains the EX2HX60-180EX2H sequence motif previously used to identify proteins with non-heme diiron clusters. Two research groups independently obtained evidence for the presence of iron and manganese in preparations of AurF. The electron paramagnetic resonance (EPR) spectrum of purified, resting AurF presented in one of these studies is markedly similar to the spectrum of the MnIII/FeIII form of C. trachomatis R2. We propose that S. thioluteus AurF also may harbor a heterodinuclear Mn/Fe cofactor, which it may use to activate O2 for oxidation of the aryl amine to the nitro compound. Hypothetical proteins encoded in the genomes of several other bacteria have similar sequences and may also be members of this nascent family of oxygen-activating Mn/Fe proteins.",
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AurF from Streptomyces thioluteus and a possible new family of manganese/iron oxygenases. / Krebs, Carsten; Matthews, Megan L.; Jiang, Wei; Bollinger, Jr., Joseph M.

In: Biochemistry, Vol. 46, No. 37, 18.09.2007, p. 10413-10418.

Research output: Contribution to journalArticle

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