Autoantibodies against RNA polymerase I in scleroderma and Sjögren's syndrome scera

Dean A. Stetler, Morris Reichlin, Cheston M. Berlin, Samson T. Jacob

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Anti-RNA polymerase I antibodies were detected by radioimmunoassay in the sera of 8 out of 9 Sjögren's syndrome patients, 11 out of 19 individuals with scleroderma, and 19 out of 19 systemic lupus erythematosus patients. The results of the radioimmunoassay were confirmed by demonstrating the ability of the patients' IgG to inhibit RNA polymerase I activity in vitro. Sera from four patients in each category were also tested for reaction with individual subunits of RNA polymerase I. All 4 SLE sera, 3 out of 4 scleroderma sera and 1 out of 4 Sjögren's syndrome sera contained antibodies against the 65 kDa (S3) subunit of RNA polymerase I in addition to antibodies against one other subunit. Sera from the remaining scleroderma and Sjögren's syndrome patients tested in this assay contained only anti-S3 antibodies. These results demonstrate that anti-RNA polymerase I antibodies are characteristic of a variety of rheumatic autoimmune sera.

Original languageEnglish (US)
Pages (from-to)1296-1302
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume144
Issue number3
DOIs
StatePublished - May 14 1987

Fingerprint

RNA Polymerase I
Autoantibodies
Antibodies
Serum
Radioimmunoassay
Assays
Immunoglobulin G
Systemic Lupus Erythematosus
Anti-Idiotypic Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Stetler, Dean A. ; Reichlin, Morris ; Berlin, Cheston M. ; Jacob, Samson T. / Autoantibodies against RNA polymerase I in scleroderma and Sjögren's syndrome scera. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 144, No. 3. pp. 1296-1302.
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abstract = "Anti-RNA polymerase I antibodies were detected by radioimmunoassay in the sera of 8 out of 9 Sj{\"o}gren's syndrome patients, 11 out of 19 individuals with scleroderma, and 19 out of 19 systemic lupus erythematosus patients. The results of the radioimmunoassay were confirmed by demonstrating the ability of the patients' IgG to inhibit RNA polymerase I activity in vitro. Sera from four patients in each category were also tested for reaction with individual subunits of RNA polymerase I. All 4 SLE sera, 3 out of 4 scleroderma sera and 1 out of 4 Sj{\"o}gren's syndrome sera contained antibodies against the 65 kDa (S3) subunit of RNA polymerase I in addition to antibodies against one other subunit. Sera from the remaining scleroderma and Sj{\"o}gren's syndrome patients tested in this assay contained only anti-S3 antibodies. These results demonstrate that anti-RNA polymerase I antibodies are characteristic of a variety of rheumatic autoimmune sera.",
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Autoantibodies against RNA polymerase I in scleroderma and Sjögren's syndrome scera. / Stetler, Dean A.; Reichlin, Morris; Berlin, Cheston M.; Jacob, Samson T.

In: Biochemical and Biophysical Research Communications, Vol. 144, No. 3, 14.05.1987, p. 1296-1302.

Research output: Contribution to journalArticle

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