Automated actin filament segmentation, tracking and tip elongation measurements based on open active contour models

Hongsheng Li, Tian Shen, Matthew B. Smith, Ikuko Fujiwara, Dimitrios Vavylonis, Xiaolei Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

40 Scopus citations

Abstract

This paper presents an automated method for actin filament segmentation and tracking for measuring tip elongation rates in Total Internal Reflection Fluorescence Microscopy (TIRFM) images. The main contributions of the paper are: (i) we use a novel open active contour model for filament segmentation and tracking, which is fast and robust against noise; and (ii) different strategies are proposed to solve the filament intersection problem, which is shown to be the main difficulty in filament tracking. Application to experimental results demonstrated the robustness and effectiveness of this method.

Original languageEnglish (US)
Title of host publicationProceedings - 2009 IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2009
Pages1302-1305
Number of pages4
DOIs
Publication statusPublished - Nov 17 2009
Event2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 - Boston, MA, United States
Duration: Jun 28 2009Jul 1 2009

Publication series

NameProceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009

Other

Other2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
CountryUnited States
CityBoston, MA
Period6/28/097/1/09

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Li, H., Shen, T., Smith, M. B., Fujiwara, I., Vavylonis, D., & Huang, X. (2009). Automated actin filament segmentation, tracking and tip elongation measurements based on open active contour models. In Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 (pp. 1302-1305). [5193303] (Proceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009). https://doi.org/10.1109/ISBI.2009.5193303