Automated microfluidic processing platform for multiplexed magnetic bead immunoassays

Lawrence A. Sasso; Ian H. Johnston; Mingde Zheng; Rohit K. Gupte; Akif Ündar; Jeffrey D. Zahn

doi:10.1007/s10404-012-0980-0

Automated microfluidic processing platform for multiplexed magnetic bead immunoassays

Lawrence A. Sasso, Ian H. Johnston, Mingde Zheng, Rohit K. Gupte, Akif Ündar, Jeffrey D. Zahn

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A microfluidic platform is presented which fully automates all incubation steps of a three-stage, multiplexed magnetic bead immunoassay, such as the Luminex® xMAP technology. Magnetic actuation is used to transfer the microbeads between co-infused adjacent laminar flow streams to transport the beads into and out of incubation and wash solutions, with extended incubation channels to allow sufficient bead incubation times (1-30 min, commonly 5 min per stage) to enable high-sensitivity. The serial incubation steps of the immunoassay are completed in succession within the device with no operator interaction, and the continuous flow operation with magnetic bead transfer defines the incubation sequencing requiring no external fluidic controls beyond syringe pump infusion. The binding kinetics of the assay is empirically characterized to determine the required incubation times for specific assay sensitivities in the range 1 pg/ml to 100 ng/ml. By using a Luminex ® xMAP duplex assay, concurrent detection of IL-6 and TNF-α was demonstrated on-chip with a detection range 10 pg/ml to 1 ng/ml. This technology enables rapid automation of magnetic microbead assays, and has the potential to perform continuous concentration monitoring.

Original language	English (US)
Pages (from-to)	603-612
Number of pages	10
Journal	Microfluidics and Nanofluidics
Volume	13
Issue number	4
DOIs	https://doi.org/10.1007/s10404-012-0980-0
State	Published - Oct 2012

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Materials Chemistry

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10.1007/s10404-012-0980-0

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title = "Automated microfluidic processing platform for multiplexed magnetic bead immunoassays",

abstract = "A microfluidic platform is presented which fully automates all incubation steps of a three-stage, multiplexed magnetic bead immunoassay, such as the Luminex{\textregistered} xMAP technology. Magnetic actuation is used to transfer the microbeads between co-infused adjacent laminar flow streams to transport the beads into and out of incubation and wash solutions, with extended incubation channels to allow sufficient bead incubation times (1-30 min, commonly 5 min per stage) to enable high-sensitivity. The serial incubation steps of the immunoassay are completed in succession within the device with no operator interaction, and the continuous flow operation with magnetic bead transfer defines the incubation sequencing requiring no external fluidic controls beyond syringe pump infusion. The binding kinetics of the assay is empirically characterized to determine the required incubation times for specific assay sensitivities in the range 1 pg/ml to 100 ng/ml. By using a Luminex {\textregistered} xMAP duplex assay, concurrent detection of IL-6 and TNF-α was demonstrated on-chip with a detection range 10 pg/ml to 1 ng/ml. This technology enables rapid automation of magnetic microbead assays, and has the potential to perform continuous concentration monitoring.",

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note = "Funding Information: Acknowledgments This work was funded by grants from the New Jersey Commission on Spinal Cord Research, the National Institutes of Health Biotechnology Pre-doctoral Training Program, the National Heart Lung Blood Institute, the Wallace H. Coulter Foundation Early Career Translational Research Awards in Biomedical Engineering, and the NIH funded Point-of-Care Center for Emerging Neuro-Technologies.",

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AU - Zahn, Jeffrey D.

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N2 - A microfluidic platform is presented which fully automates all incubation steps of a three-stage, multiplexed magnetic bead immunoassay, such as the Luminex® xMAP technology. Magnetic actuation is used to transfer the microbeads between co-infused adjacent laminar flow streams to transport the beads into and out of incubation and wash solutions, with extended incubation channels to allow sufficient bead incubation times (1-30 min, commonly 5 min per stage) to enable high-sensitivity. The serial incubation steps of the immunoassay are completed in succession within the device with no operator interaction, and the continuous flow operation with magnetic bead transfer defines the incubation sequencing requiring no external fluidic controls beyond syringe pump infusion. The binding kinetics of the assay is empirically characterized to determine the required incubation times for specific assay sensitivities in the range 1 pg/ml to 100 ng/ml. By using a Luminex ® xMAP duplex assay, concurrent detection of IL-6 and TNF-α was demonstrated on-chip with a detection range 10 pg/ml to 1 ng/ml. This technology enables rapid automation of magnetic microbead assays, and has the potential to perform continuous concentration monitoring.

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Automated microfluidic processing platform for multiplexed magnetic bead immunoassays

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