Autoregulation of 2μm circle gene expression provides a model for maintenance of stable plasmid copy levels

Tapan Som, Karen A. Armstrong, Frederic C. Volkert, James Broach

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The yeast plasmid 2μm circle actively maintains high but stable copy levels in the cell, even though the plasmid confers no selective advantage to its host. To address the mechanism by which stable copy control is achieved, we have examined the level of expression of the genes resident on the yeast plasmid 2μm circle as a function of the presence of proteins encoded by the plasmid. We find that transcription of the site-specific recombinase gene, FLP, is repressed at least 100-fold by the concerted action of the products of two other plasmid genes, REP1 and REP2. In addition, these products repress transcription of the REP1 gene itself. These results can be formulated into a consistent model for plasmid copy control.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalCell
Volume52
Issue number1
DOIs
StatePublished - Jan 15 1988

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Gene expression
Plasmids
Homeostasis
Maintenance
Gene Expression
Genes
Transcription
Yeast
Yeasts
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Som, Tapan ; Armstrong, Karen A. ; Volkert, Frederic C. ; Broach, James. / Autoregulation of 2μm circle gene expression provides a model for maintenance of stable plasmid copy levels. In: Cell. 1988 ; Vol. 52, No. 1. pp. 27-37.
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Autoregulation of 2μm circle gene expression provides a model for maintenance of stable plasmid copy levels. / Som, Tapan; Armstrong, Karen A.; Volkert, Frederic C.; Broach, James.

In: Cell, Vol. 52, No. 1, 15.01.1988, p. 27-37.

Research output: Contribution to journalArticle

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