Axenic aerobic biofilms inhibit corrosion of SAE 1018 steel through oxygen depletion

A. Jayaraman, E. T. Cheng, J. C. Earthman, Thomas Keith Wood

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Corrosion inhibition of SAE 1018 steel by pure-culture biofilms of Pseudomonas fragi and Escherichia coli DH5α has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modified Baar's medium at 30 °C. In batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. To corroborate this result, a continuous reactor and electrochemical impedance spectroscopy were used to show that both P. fragi K and E. coli DH5α decreased the corrosion rate by 4- to 40- fold as compared to sterile controls; this matched the decrease in corrosion found with sterile medium in the absence of oxygen and with E. coli DH5α grown anaerobically. In addition, the requirement for live respiring cells was demonstrated by the increase in the corrosion rate that was observed upon killing the P. fragi K biofilm in continuous cultures, and it was shown that fermentation products do not cause an increase in corrosion. Hence, pure- culture biofilms inhibit corrosion of SAE 1018 steel by depleting oxygen at the metal surface.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume48
Issue number1
DOIs
StatePublished - Sep 8 1997

Fingerprint

Corrosion
Steel
Biofilms
Oxygen
Pseudomonas fragi
Escherichia coli
Dielectric Spectroscopy
Batch Cell Culture Techniques
Seawater
Fermentation
Metals
Bacteria

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Corrosion inhibition of SAE 1018 steel by pure-culture biofilms of Pseudomonas fragi and Escherichia coli DH5α has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modified Baar's medium at 30 °C. In batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. To corroborate this result, a continuous reactor and electrochemical impedance spectroscopy were used to show that both P. fragi K and E. coli DH5α decreased the corrosion rate by 4- to 40- fold as compared to sterile controls; this matched the decrease in corrosion found with sterile medium in the absence of oxygen and with E. coli DH5α grown anaerobically. In addition, the requirement for live respiring cells was demonstrated by the increase in the corrosion rate that was observed upon killing the P. fragi K biofilm in continuous cultures, and it was shown that fermentation products do not cause an increase in corrosion. Hence, pure- culture biofilms inhibit corrosion of SAE 1018 steel by depleting oxygen at the metal surface.",
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Axenic aerobic biofilms inhibit corrosion of SAE 1018 steel through oxygen depletion. / Jayaraman, A.; Cheng, E. T.; Earthman, J. C.; Wood, Thomas Keith.

In: Applied Microbiology and Biotechnology, Vol. 48, No. 1, 08.09.1997, p. 11-17.

Research output: Contribution to journalArticle

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