Backbone dynamics and hydrogen exchange of Pseudomonas aeruginosa ferricytochrome C551

Brandy S. Russell, Linghao Zhong, Maria Giulia Bigotti, Francesca Cutruzzolà, Kara L. Bren

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38 Scopus citations

Abstract

A model-free analysis of Pseudomonas aeruginosa ferricytochrome C551 dynamics based on 15N R1 15N R2 and {1H}-15N heteronuclear nuclear Overhauser effect data is reported. The protein backbone is highly rigid (<S2> = 0.924±0.005) and displays little variation in picosecond-nanosecond time scale dynamics over the structure. The loop structure containing the axial methionine ligand (loop 3) displays anomalous rigidity, which is attributed to its high proline content. Also reported are protection factors calculated from hydrogen-exchange rates. These data reveal that loop 3 residues, including the axial methionine, are protected from exchange as a result of long-range hydrogen-bonding interactions. These results are contrasted with data reported for Saccharomyces cerevisiae iso-1-ferricytochrome c, which displays higher overall flexibility (<S2> = 0.80±0.07), greater variation of dynamics as a function of structure, and low protection factors for loop 3. This analysis reveals that heme proteins with similar functions and topologies may display diverse dynamical properties. Electronic supplementary material is available if you access this article at http://dx.doi.org/10.1007/s00775-002-0401-z. On that page (frame on the left side), a link takes you directly to the supplementary material.

Original languageEnglish (US)
Pages (from-to)156-166
Number of pages11
JournalJournal of Biological Inorganic Chemistry
Volume8
Issue number1-2
DOIs
StatePublished - Jan 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

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