Baclofen inhibits with high affinity an L-type - like voltage-dependent calcium channel in cerebellar granule cell cultures

W. J. Wojcik, Renato Alberto Travagli, E. Costa, M. Bertolino

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In primary cultures of cerebellar granule cells, D,L baclofen (p-chlorophenyl-GABA) inhibited appoximately 50% of the calcium-45 influx induced with cell depolarization. The half maximal effective concentration for baclofen was 4 nM. Basal calcium influx was not influenced by baclofen thus suggesting that its inhibitory action could be exerted via a voltage dependent calcium channel (VDCC). Whole-cell recordings by patch-clamp technique showed a calcium current that appeared to be similar to the reported L-type VDCC. Nanomolar concentrations of baclofen also inhibited this calcium current by about 60%. However, in order for baclofen to be active, it needed to be placed into the incubation buffer at least five minutes before patching a cell raising the possibility that baclofen may be acting to inhibit the VDCC via a second messenger system.

Original languageEnglish (US)
Pages (from-to)969-972
Number of pages4
JournalNeuropharmacology
Volume29
Issue number10
DOIs
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Baclofen inhibits with high affinity an L-type - like voltage-dependent calcium channel in cerebellar granule cell cultures'. Together they form a unique fingerprint.

  • Cite this