TY - CONF
T1 - Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS)
AU - Iram, Attia
AU - Cekmecelioglu, Deniz
AU - Demirci, Ali
N1 - Funding Information:
This work was supported in part by FULBRIGHT Student Program by providing scholarship to Attia Iram, USDA Northeast Sun Grant Initiative (NE-SGI) Competitive Grants Program, and the USDA National Institute of Food and Agriculture Federal Appropriations under Project PEN04594 and Accession number 1007291. The authors also gratefully acknowledge Pennsylvania Grain Processing, LLC® (Clearfield, PA, USA) for providing the DDGS.
Publisher Copyright:
© ASABE 2020 Annual International Meeting.
PY - 2020
Y1 - 2020
N2 - Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done using DDGS as the feedstock for the hydrolytic enzyme production. Among all these strains, Aspergillus niger (NRRL 1956) produced the maximum cellulase of 0.592 IU/ml. On the other hand, stable high production of xylanase of 34.8 IU/ml was obtained by Aspergillus niger (NRRL 567) on day twelfth. Bacterial enzyme production by Bacillus subtilis (NRRL NSR352, DSM 17766, and NF1) was relatively lower (0-0.261 IU/ml for cellulase and 1.2-5.2 IU/ml for xylanase) than the fungal enzyme production. In conclusion, this study demonstrated that the hydrolyzed DDGS can be an alternative economical substrate for A. niger strains to produce cellulase and xylanase, respectively. The next phases of the study should further optimize the production of cellulase and xylanase in terms of growth parameters and medium components by using benchtop bioreactors.
AB - Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done using DDGS as the feedstock for the hydrolytic enzyme production. Among all these strains, Aspergillus niger (NRRL 1956) produced the maximum cellulase of 0.592 IU/ml. On the other hand, stable high production of xylanase of 34.8 IU/ml was obtained by Aspergillus niger (NRRL 567) on day twelfth. Bacterial enzyme production by Bacillus subtilis (NRRL NSR352, DSM 17766, and NF1) was relatively lower (0-0.261 IU/ml for cellulase and 1.2-5.2 IU/ml for xylanase) than the fungal enzyme production. In conclusion, this study demonstrated that the hydrolyzed DDGS can be an alternative economical substrate for A. niger strains to produce cellulase and xylanase, respectively. The next phases of the study should further optimize the production of cellulase and xylanase in terms of growth parameters and medium components by using benchtop bioreactors.
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U2 - 10.13031/aim.202000032
DO - 10.13031/aim.202000032
M3 - Paper
AN - SCOPUS:85096618650
T2 - 2020 ASABE Annual International Meeting
Y2 - 13 July 2020 through 15 July 2020
ER -