Bacterial community evaluation during establishment of tall fescue (Festuca arundinacea) in soil contaminated with pyrene

Yen-chih Chen, M. Katherine Banks

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A greenhouse study was conducted to monitor microbial community dynamics related to contaminant concentration and plant growth during the phytoremediation of pyrene. Soil microbial communities in both bulk and rhizosphere soils were assessed using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). However, no correlation between pyrene concentration and bacterial community shifts was observed. Even though plants significantly enhanced pyrene degradation (undetectable after 91 d for planted treatments compared with 82 mg/kg for unplanted treatments after 147 d with initial concentration of 758 mg/kg), biodegradation may have been accomplished by the pre-existing microbial community. It is also possible that the method was unable to pick up subtle community shifts, considering that the carbon source from pyrene was only 3.7% of the existing soil organic matter. This research suggests that plants significantly enhance degradation and mineralization of pyrene in soil, although there was no conclusive evidence supporting any specific bacterial activity responsible for this enhancement.

Original languageEnglish (US)
Pages (from-to)227-238
Number of pages12
JournalInternational Journal of Phytoremediation
Volume6
Issue number3
DOIs
StatePublished - Oct 18 2004

Fingerprint

Festuca
Pyrene
Festuca arundinacea
pyrene
bacterial communities
polluted soils
microbial communities
Soil
Soils
microbial community
soil
degradation
phytoremediation
denaturing gradient gel electrophoresis
biodegradation
microbial activity
rhizosphere
mineralization
soil organic matter
Degradation

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution
  • Plant Science

Cite this

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abstract = "A greenhouse study was conducted to monitor microbial community dynamics related to contaminant concentration and plant growth during the phytoremediation of pyrene. Soil microbial communities in both bulk and rhizosphere soils were assessed using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). However, no correlation between pyrene concentration and bacterial community shifts was observed. Even though plants significantly enhanced pyrene degradation (undetectable after 91 d for planted treatments compared with 82 mg/kg for unplanted treatments after 147 d with initial concentration of 758 mg/kg), biodegradation may have been accomplished by the pre-existing microbial community. It is also possible that the method was unable to pick up subtle community shifts, considering that the carbon source from pyrene was only 3.7{\%} of the existing soil organic matter. This research suggests that plants significantly enhance degradation and mineralization of pyrene in soil, although there was no conclusive evidence supporting any specific bacterial activity responsible for this enhancement.",
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Bacterial community evaluation during establishment of tall fescue (Festuca arundinacea) in soil contaminated with pyrene. / Chen, Yen-chih; Banks, M. Katherine.

In: International Journal of Phytoremediation, Vol. 6, No. 3, 18.10.2004, p. 227-238.

Research output: Contribution to journalArticle

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