Bacterial FtsZ protein forms phase-separated condensates with its nucleoid-associated inhibitor SlmA

Begoña Monterroso, Silvia Zorrilla, Marta Sobrinos-Sanguino, Miguel A. Robles-Ramos, Marina López-Álvarez, William Margolin, Christine D. Keating, Germán Rivas

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Macromolecular condensation resulting from biologically regulated liquid–liquid phase separation is emerging as a mechanism to organize intracellular space in eukaryotes, with broad implications for cell physiology and pathology. Despite their small size, bacterial cells are also organized by proteins such as FtsZ, a tubulin homolog that assembles into a ring structure precisely at the cell midpoint and is required for cytokinesis. Here, we demonstrate that FtsZ can form crowding-induced condensates, reminiscent of those observed for eukaryotic proteins. Formation of these FtsZ-rich droplets occurs when FtsZ is bound to SlmA, a spatial regulator of FtsZ that antagonizes polymerization, while also binding to specific sites on chromosomal DNA. The resulting condensates are dynamic, allowing FtsZ to undergo GTP-driven assembly to form protein fibers. They are sensitive to compartmentalization and to the presence of a membrane boundary in cell mimetic systems. This is a novel example of a bacterial nucleoprotein complex exhibiting condensation into liquid droplets, suggesting that phase separation may also play a functional role in the spatiotemporal organization of essential bacterial processes.

Original languageEnglish (US)
Article numbere45946
JournalEMBO Reports
Volume20
Issue number1
DOIs
StatePublished - Jan 2019

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Monterroso, B., Zorrilla, S., Sobrinos-Sanguino, M., Robles-Ramos, M. A., López-Álvarez, M., Margolin, W., Keating, C. D., & Rivas, G. (2019). Bacterial FtsZ protein forms phase-separated condensates with its nucleoid-associated inhibitor SlmA. EMBO Reports, 20(1), [e45946]. https://doi.org/10.15252/embr.201845946