Bacteriochlorophyllide c C-82 and C-121 methyltransferases are essential for adaptation to low light in Chlorobaculum tepidum

Aline Gomez Maqueo Chew, Niels Ulrik Frigaard, Donald A. Bryant

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79 Scopus citations

Abstract

Bacteriochlorophyll (BChl) c is the major photosynthetic pigment in the green sulfur bacterium Chlorobaculum tepidum, in which it forms protein-independent aggregates that function in light harvesting. BChls c, d, and e are found only in chlorosome-producing bacteria and are unique among chlorophylls because of methylations that occur at the C-82 and C-121 carbons. Two genes required for these methylation reactions were identified and designated bchQ (CT1777) and bchR (CT1320). BchQ and BchR are members of the radical S-adenosyl-methionine (SAM) protein superfamily; each has sequence motifs to ligate a [4Fe-4S] cluster, and we propose that they catalyze the methyl group transfers. bchQ, bchR, and bchQ bchR mutants of C. tepidum were constructed and characterized. The bchQ mutant produced BChl c that was not methylated at C-82, the bchR mutant produced BChl c that was not methylated at C-121, and the double mutant produced [8-ethyl, 12-methyl]-BChl c that lacked methylation at both the C-82 and C-121 positions. Compared to the wild type, the Qy absorption bands for BChl c in the mutant cells were narrower and blue shifted to various extents. All three mutants grew slower and had a lower cellular BChl c content than the wild type, an effect that was especially pronounced at low light intensities. These observations show that the C-82 and C-121 methylations of BChl c play important roles in the adaptation of C. tepidum to low light intensity. The data additionally suggest that these methylations also directly or indirectly affect the regulation of the BChl c biosynthetic pathway.

Original languageEnglish (US)
Pages (from-to)6176-6184
Number of pages9
JournalJournal of bacteriology
Volume189
Issue number17
DOIs
StatePublished - Sep 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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