Basophil influx occurs after nasal antigen challenge: Effects of topical corticosteroid pretreatment

R. Bascom, M. Wachs, R. M. Naclerio, U. Pipkorn, S. J. Galli, L. M. Lichtenstein

Research output: Contribution to journalArticle

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Abstract

Both the pattern of mediator release during the late-phase response (LPR) and the reduction of the LPR with corticosteroid pretreatment have suggested that basophils, not mast cells, represent the main source of histamine in the late response to nasal antigen challenge. We tested this hypothesis by examining alcian blue-stained cytospin slides of nasal washings obtained before and for 11 hours after nasal antigen challenge in 11 asymptomatic subjects with seasonal allergic rhinitis. In a double-blind manner, subjects received placebo or topical flunisolide (50 μg, each nostril, twice daily) for 1 week before antigen challenge. One month later, the challenge was repeated with the alternate pretreatment. On placebo-treatment days, a twelvefold increase occurred in the number and a threefold increase in the percentage of alcian blue-stained positive cells in nasal washings in the LPR compared to baseline. At least 68% of these alcian blue-stained positive cells were basophils, as determined by light microscopic criteria. Alcian blue-stained cell influx correlated with increases in histamine levels in nasal washes (p < 0.001). Topical steroid pretreatment blocked the influx of alcian blue-stained positive cells, as well as other inflammatory cells, including eosinophils, neutrophils, and mononuclear cells. Symptoms and mediator release were also blocked. These data demonstrate an influx of basophils and suggest that these cells are responsible for the histamine release observed in the LPR. Our findings indicate that pharmacologic control of basophil histamine release may represent a strategy for the treatment of a variety of chronic allergic diseases that are believed to resemble the LPR.

Original languageEnglish (US)
Pages (from-to)580-589
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume81
Issue number3
DOIs
StatePublished - Jan 1 1988

Fingerprint

Basophils
Nose
Alcian Blue
Adrenal Cortex Hormones
Antigens
Nasal Lavage Fluid
flunisolide
Histamine Release
Histamine
Placebos
Seasonal Allergic Rhinitis
Eosinophils
Mast Cells
Neutrophils
Chronic Disease
Steroids
Light
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Bascom, R. ; Wachs, M. ; Naclerio, R. M. ; Pipkorn, U. ; Galli, S. J. ; Lichtenstein, L. M. / Basophil influx occurs after nasal antigen challenge : Effects of topical corticosteroid pretreatment. In: Journal of Allergy and Clinical Immunology. 1988 ; Vol. 81, No. 3. pp. 580-589.
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Basophil influx occurs after nasal antigen challenge : Effects of topical corticosteroid pretreatment. / Bascom, R.; Wachs, M.; Naclerio, R. M.; Pipkorn, U.; Galli, S. J.; Lichtenstein, L. M.

In: Journal of Allergy and Clinical Immunology, Vol. 81, No. 3, 01.01.1988, p. 580-589.

Research output: Contribution to journalArticle

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