Bcl-2, Raf-1 and mitochondrial regulation of apoptosis

Hong-Gang Wang, John C. Reed

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Raf-1 kinase was shown to bind via its catalytic domain (Cat) to Bcl-2 in a BH4 domain-dependent manner. Using a green fluorescent protein (GFP)- Raf-1 (Cat) fusion protein, Bcl-2 but not Bcl-2(ΔBH4) was found to target Raf-1 to mitochondria in cells. Targeting Raf-1 (Cat) to mitochondrial membranes by fusing with the transmembrane domain of an outer mitochondrial membrane protein protected cells from apoptosis and resulted in phosphorylation of BAD protein, whereas plasma-membrane targeted Raf-1 failed to phosphorylate BAD and did not protect against cell death. Moreover, a Bcl- 2 binding protein, BAG-1, was shown to not only bind Raf-1 but also increase the activity of this kinase through a protein-protein interaction. The findings suggest that Bcl-2 targets Raf-1 to mitochondria, allowing this kinase to contribute to cellular survival by phosphorylating BAD or possibly other protein substrates in the vicinity of Bcl-2.

Original languageEnglish (US)
Pages (from-to)13-16
Number of pages4
JournalBioFactors
Volume8
Issue number1-2
DOIs
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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