Bell pepper endornavirus: Molecular and biological properties, and occurrence in the genus Capsicum

Ryo Okada, Eri Kiyota, Sead Sabanadzovic, Hiromitsu Moriyama, Toshiyuki Fukuhara, Prasenjit Saha, Marilyn J. Roossinck, Ake Severin, Rodrigo A. Valverde

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Bell peppers (Capsicum annuum) harbour a large dsRNA virus. The linear genome (14.7 kbp) of two isolates from Japanese and USA bell pepper cultivars were completely sequenced and compared. They shared extensive sequence identity and contained a single, long ORF encoding a 4815 aa protein. This polyprotein contained conserved motifs of putative viral methyltransferase (MTR), helicase 1 (Hel-1), UDP-glycosyltransferase and RNA-dependent RNA polymerase. This unique arrangement of conserved domains has not been reported in any of the known endornaviruses. Hence this virus, for which the name Bell pepper endornavirus (BPEV) is proposed, is a distinct species in the genus Endornavirus (family Endornaviridae). The BPEVencoded polyprotein contains a cysteine-rich region between the MTR and Hel-1 domains, with conserved CXCC motifs shared among several endornaviruses, suggesting an additional functional domain. In agreement with general endornavirus features, BPEV contains a nick in the positive-strand RNA molecule. The virus was detected in all bell pepper cultivars tested and transmitted through seed but not by graft inoculations. Analysis of dsRNA patterns and RT-PCR using degenerate primers revealed putative variants of BPEV, or closely related species, infecting other C. annuum genotypes and three other Capsicum species (C. baccatum, C. chinense and C. frutescens).

Original languageEnglish (US)
Pages (from-to)2664-2673
Number of pages10
JournalJournal of General Virology
Volume92
Issue number11
DOIs
StatePublished - Nov 1 2011

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Capsicum
Polyproteins
Methyltransferases
Viruses
RNA Replicase
Glycosyltransferases
Uridine Diphosphate
Open Reading Frames
Names
Cysteine
Seeds
Genotype
Genome
RNA
Transplants
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Okada, R., Kiyota, E., Sabanadzovic, S., Moriyama, H., Fukuhara, T., Saha, P., ... Valverde, R. A. (2011). Bell pepper endornavirus: Molecular and biological properties, and occurrence in the genus Capsicum. Journal of General Virology, 92(11), 2664-2673. https://doi.org/10.1099/vir.0.034686-0
Okada, Ryo ; Kiyota, Eri ; Sabanadzovic, Sead ; Moriyama, Hiromitsu ; Fukuhara, Toshiyuki ; Saha, Prasenjit ; Roossinck, Marilyn J. ; Severin, Ake ; Valverde, Rodrigo A. / Bell pepper endornavirus : Molecular and biological properties, and occurrence in the genus Capsicum. In: Journal of General Virology. 2011 ; Vol. 92, No. 11. pp. 2664-2673.
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Okada, R, Kiyota, E, Sabanadzovic, S, Moriyama, H, Fukuhara, T, Saha, P, Roossinck, MJ, Severin, A & Valverde, RA 2011, 'Bell pepper endornavirus: Molecular and biological properties, and occurrence in the genus Capsicum', Journal of General Virology, vol. 92, no. 11, pp. 2664-2673. https://doi.org/10.1099/vir.0.034686-0

Bell pepper endornavirus : Molecular and biological properties, and occurrence in the genus Capsicum. / Okada, Ryo; Kiyota, Eri; Sabanadzovic, Sead; Moriyama, Hiromitsu; Fukuhara, Toshiyuki; Saha, Prasenjit; Roossinck, Marilyn J.; Severin, Ake; Valverde, Rodrigo A.

In: Journal of General Virology, Vol. 92, No. 11, 01.11.2011, p. 2664-2673.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bell pepper endornavirus

T2 - Molecular and biological properties, and occurrence in the genus Capsicum

AU - Okada, Ryo

AU - Kiyota, Eri

AU - Sabanadzovic, Sead

AU - Moriyama, Hiromitsu

AU - Fukuhara, Toshiyuki

AU - Saha, Prasenjit

AU - Roossinck, Marilyn J.

AU - Severin, Ake

AU - Valverde, Rodrigo A.

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Bell peppers (Capsicum annuum) harbour a large dsRNA virus. The linear genome (14.7 kbp) of two isolates from Japanese and USA bell pepper cultivars were completely sequenced and compared. They shared extensive sequence identity and contained a single, long ORF encoding a 4815 aa protein. This polyprotein contained conserved motifs of putative viral methyltransferase (MTR), helicase 1 (Hel-1), UDP-glycosyltransferase and RNA-dependent RNA polymerase. This unique arrangement of conserved domains has not been reported in any of the known endornaviruses. Hence this virus, for which the name Bell pepper endornavirus (BPEV) is proposed, is a distinct species in the genus Endornavirus (family Endornaviridae). The BPEVencoded polyprotein contains a cysteine-rich region between the MTR and Hel-1 domains, with conserved CXCC motifs shared among several endornaviruses, suggesting an additional functional domain. In agreement with general endornavirus features, BPEV contains a nick in the positive-strand RNA molecule. The virus was detected in all bell pepper cultivars tested and transmitted through seed but not by graft inoculations. Analysis of dsRNA patterns and RT-PCR using degenerate primers revealed putative variants of BPEV, or closely related species, infecting other C. annuum genotypes and three other Capsicum species (C. baccatum, C. chinense and C. frutescens).

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