Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors

U. Gunther, J. Benson, D. Benke, J. M. Fritschy, G. Reyes, F. Knoflach, F. Crestani, A. Aguzzi, M. Arigoni, Y. Lang, H. Bluethmann, H. Mohler, Bernhard Luscher

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Abstract

Vigilance, anxiety, epileptic activity, and muscle tone can be modulated by drugs acting at the benzodiazepine (BZ) site of γ-aminobutyric acid type A (GABA(A)) receptors. In vivo, BZ sites are potential targets for endogenous ligands regulating the corresponding central nervous system states. To assess the physiological relevance of BZ sites, mice were generated containing GABA(A) receptors devoid of BZ sites. Following targeted disruption of the γ2 subunit gene, 94% of the BZ sites were absent in brain of neonatal mice, while the number of GABA sites was only slightly reduced. Except for the γ2 subunit, the level of expression and the regional and cellular distribution of the major GABA(A) receptor subunits were unaltered. The single channel main conductance level and the Hill coefficient were reduced to values consistent with recombinant GABA(A) receptors composed of α and β subunits. The GABA response was potentiated by pentobarbital but not by flunitrazepam. Diazepam was inactive behaviorally. Thus, the γ2 subunit is dispensable for the assembly of functional GABA(A) receptors but is required for normal channel conductance and the formation of BZ sites in vivo. BZ sites are not essential for embryonic development, as suggested by the normal body weight and histology of newborn mice. Postnatally, however, the reduced GABA(A) receptor function is associated with retarded growth, sensorimotor dysfunction, and drastically reduced life-span. The lack of postnatal GABA(A) receptor regulation by endogenous ligands of BZ sites might contribute to this phenotype.

Original languageEnglish (US)
Pages (from-to)7749-7753
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number17
DOIs
StatePublished - Aug 31 1995

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Aminobutyrates
Benzodiazepines
GABA-A Receptors
Genes
gamma-Aminobutyric Acid
Ligands
Flunitrazepam
Ideal Body Weight
Pentobarbital
Diazepam
Embryonic Development
Histology
Anxiety
Central Nervous System
Phenotype
Muscles
Brain

All Science Journal Classification (ASJC) codes

  • General

Cite this

Gunther, U. ; Benson, J. ; Benke, D. ; Fritschy, J. M. ; Reyes, G. ; Knoflach, F. ; Crestani, F. ; Aguzzi, A. ; Arigoni, M. ; Lang, Y. ; Bluethmann, H. ; Mohler, H. ; Luscher, Bernhard. / Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 17. pp. 7749-7753.
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abstract = "Vigilance, anxiety, epileptic activity, and muscle tone can be modulated by drugs acting at the benzodiazepine (BZ) site of γ-aminobutyric acid type A (GABA(A)) receptors. In vivo, BZ sites are potential targets for endogenous ligands regulating the corresponding central nervous system states. To assess the physiological relevance of BZ sites, mice were generated containing GABA(A) receptors devoid of BZ sites. Following targeted disruption of the γ2 subunit gene, 94{\%} of the BZ sites were absent in brain of neonatal mice, while the number of GABA sites was only slightly reduced. Except for the γ2 subunit, the level of expression and the regional and cellular distribution of the major GABA(A) receptor subunits were unaltered. The single channel main conductance level and the Hill coefficient were reduced to values consistent with recombinant GABA(A) receptors composed of α and β subunits. The GABA response was potentiated by pentobarbital but not by flunitrazepam. Diazepam was inactive behaviorally. Thus, the γ2 subunit is dispensable for the assembly of functional GABA(A) receptors but is required for normal channel conductance and the formation of BZ sites in vivo. BZ sites are not essential for embryonic development, as suggested by the normal body weight and histology of newborn mice. Postnatally, however, the reduced GABA(A) receptor function is associated with retarded growth, sensorimotor dysfunction, and drastically reduced life-span. The lack of postnatal GABA(A) receptor regulation by endogenous ligands of BZ sites might contribute to this phenotype.",
author = "U. Gunther and J. Benson and D. Benke and Fritschy, {J. M.} and G. Reyes and F. Knoflach and F. Crestani and A. Aguzzi and M. Arigoni and Y. Lang and H. Bluethmann and H. Mohler and Bernhard Luscher",
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Gunther, U, Benson, J, Benke, D, Fritschy, JM, Reyes, G, Knoflach, F, Crestani, F, Aguzzi, A, Arigoni, M, Lang, Y, Bluethmann, H, Mohler, H & Luscher, B 1995, 'Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors', Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 17, pp. 7749-7753. https://doi.org/10.1073/pnas.92.17.7749

Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors. / Gunther, U.; Benson, J.; Benke, D.; Fritschy, J. M.; Reyes, G.; Knoflach, F.; Crestani, F.; Aguzzi, A.; Arigoni, M.; Lang, Y.; Bluethmann, H.; Mohler, H.; Luscher, Bernhard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 17, 31.08.1995, p. 7749-7753.

Research output: Contribution to journalArticle

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T1 - Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors

AU - Gunther, U.

AU - Benson, J.

AU - Benke, D.

AU - Fritschy, J. M.

AU - Reyes, G.

AU - Knoflach, F.

AU - Crestani, F.

AU - Aguzzi, A.

AU - Arigoni, M.

AU - Lang, Y.

AU - Bluethmann, H.

AU - Mohler, H.

AU - Luscher, Bernhard

PY - 1995/8/31

Y1 - 1995/8/31

N2 - Vigilance, anxiety, epileptic activity, and muscle tone can be modulated by drugs acting at the benzodiazepine (BZ) site of γ-aminobutyric acid type A (GABA(A)) receptors. In vivo, BZ sites are potential targets for endogenous ligands regulating the corresponding central nervous system states. To assess the physiological relevance of BZ sites, mice were generated containing GABA(A) receptors devoid of BZ sites. Following targeted disruption of the γ2 subunit gene, 94% of the BZ sites were absent in brain of neonatal mice, while the number of GABA sites was only slightly reduced. Except for the γ2 subunit, the level of expression and the regional and cellular distribution of the major GABA(A) receptor subunits were unaltered. The single channel main conductance level and the Hill coefficient were reduced to values consistent with recombinant GABA(A) receptors composed of α and β subunits. The GABA response was potentiated by pentobarbital but not by flunitrazepam. Diazepam was inactive behaviorally. Thus, the γ2 subunit is dispensable for the assembly of functional GABA(A) receptors but is required for normal channel conductance and the formation of BZ sites in vivo. BZ sites are not essential for embryonic development, as suggested by the normal body weight and histology of newborn mice. Postnatally, however, the reduced GABA(A) receptor function is associated with retarded growth, sensorimotor dysfunction, and drastically reduced life-span. The lack of postnatal GABA(A) receptor regulation by endogenous ligands of BZ sites might contribute to this phenotype.

AB - Vigilance, anxiety, epileptic activity, and muscle tone can be modulated by drugs acting at the benzodiazepine (BZ) site of γ-aminobutyric acid type A (GABA(A)) receptors. In vivo, BZ sites are potential targets for endogenous ligands regulating the corresponding central nervous system states. To assess the physiological relevance of BZ sites, mice were generated containing GABA(A) receptors devoid of BZ sites. Following targeted disruption of the γ2 subunit gene, 94% of the BZ sites were absent in brain of neonatal mice, while the number of GABA sites was only slightly reduced. Except for the γ2 subunit, the level of expression and the regional and cellular distribution of the major GABA(A) receptor subunits were unaltered. The single channel main conductance level and the Hill coefficient were reduced to values consistent with recombinant GABA(A) receptors composed of α and β subunits. The GABA response was potentiated by pentobarbital but not by flunitrazepam. Diazepam was inactive behaviorally. Thus, the γ2 subunit is dispensable for the assembly of functional GABA(A) receptors but is required for normal channel conductance and the formation of BZ sites in vivo. BZ sites are not essential for embryonic development, as suggested by the normal body weight and histology of newborn mice. Postnatally, however, the reduced GABA(A) receptor function is associated with retarded growth, sensorimotor dysfunction, and drastically reduced life-span. The lack of postnatal GABA(A) receptor regulation by endogenous ligands of BZ sites might contribute to this phenotype.

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