Beyond type 1 regulatory t cells: Co-expression of LAG3 and CD49b in IL-10-producing t cell lineages

Weishan Huang, Sabrina Solouki, Chavez Carter, Song Guo Zheng, Avery August

Research output: Contribution to journalArticle

Abstract

Type 1 regulatory CD4+ T (Tr1) cells express high levels of the immunosuppressive cytokine IL-10 but not the master transcription factor Foxp3, and can suppress inflammation and promote immune tolerance. In order to identify and obtain viable Tr1 cells for research and clinical applications, co-expression of CD49b and LAG3 has been proposed as a unique surface signature for both human and mouse Tr1 cells. However, recent studies have revealed that this pattern of co-expression is dependent on the stimulating conditions and the differentiation stage of the CD4+ T cells. Here, using an IL-10GFP/Foxp3RFP dual reporter transgenic murine model, we demonstrate that co-expression of CD49b and LAG3 is not restricted to the Foxp3- Tr1 cells, but is also observed in Foxp3+ T regulatory (Treg) cells and CD8+ T cells that produce IL-10. Our data indicate that IL-10-producing Tr1 cells, Treg cells and CD8+ T cells are all capable of co-expressing LAG3 and CD49b in vitro following differentiation under IL-10-inducing conditions, and in vivo following pathogenic insult or infection in the pulmonary mucosa. Our findings urge caution in the use of LAG3/CD49b co-expression as sole markers to identify Tr1 cells, since it may mark IL-10-producing T cell lineages more broadly, including the Foxp3- Tr1 cells, Foxp3+ Treg cells, and CD8+ T cells.

Original languageEnglish (US)
Article number2625
JournalFrontiers in immunology
Volume9
Issue numberNOV
DOIs
StatePublished - Nov 19 2018

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Cell Lineage
Regulatory T-Lymphocytes
Interleukin-10
T-Lymphocytes
Immune Tolerance
Immunosuppressive Agents
Mucous Membrane
Transcription Factors
Cytokines
Inflammation
Lung

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Huang, Weishan ; Solouki, Sabrina ; Carter, Chavez ; Zheng, Song Guo ; August, Avery. / Beyond type 1 regulatory t cells : Co-expression of LAG3 and CD49b in IL-10-producing t cell lineages. In: Frontiers in immunology. 2018 ; Vol. 9, No. NOV.
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Beyond type 1 regulatory t cells : Co-expression of LAG3 and CD49b in IL-10-producing t cell lineages. / Huang, Weishan; Solouki, Sabrina; Carter, Chavez; Zheng, Song Guo; August, Avery.

In: Frontiers in immunology, Vol. 9, No. NOV, 2625, 19.11.2018.

Research output: Contribution to journalArticle

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