Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance

Ying Liu, Yoshinori Takahashi, Neelam Desai, Jun Zhang, Jacob M. Serfass, Yu Guang Shi, Christopher J. Lynch, Hong-Gang Wang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bif-1 is a membrane-curvature inducing protein that is implicated in the regulation of autophagy and tumorigenesis. Here, we report that Bif-1 plays a critical role in regulating lipid catabolism to control the size of lipid droplets and prevent the development of obesity and insulin resistance upon aging or dietary challenge. Our data show that Bif-1 deficiency promotes the expansion of adipose tissue mass without altering food intake or physical activities. While Bif-1 is dispensable for adipose tissue development, its deficiency reduces the basal rate of adipose tissue lipolysis and results in adipocyte hypertrophy upon aging. The importance of Bif-1 in lipid turnover is not limited to adipose tissue since fasting and refeeding-induced lipid droplet clearance is also attenuated by Bif-1 loss in the liver. Interestingly, obesity induced by a high fat-diet or Bif-1 deficiency downregulates the expression of proteins involved in the autophagy-lysosomal pathway, including Atg9a and Lamp1 in the adipose tissue. These findings thus identify Bif-1 as a novel regulator of lipid homeostasis to prevent the pathogenesis of obesity and its associated metabolic complications.

Original languageEnglish (US)
Article number20453
JournalScientific reports
Volume6
DOIs
StatePublished - Feb 9 2016

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Insulin Resistance
Adipose Tissue
Homeostasis
Obesity
Lipids
Autophagy
Lipolysis
High Fat Diet
Adipocytes
Hypertrophy
Fasting
Carcinogenesis
Proteins
Down-Regulation
Eating
Membranes
Liver
Lipid Droplets

All Science Journal Classification (ASJC) codes

  • General

Cite this

Liu, Ying ; Takahashi, Yoshinori ; Desai, Neelam ; Zhang, Jun ; Serfass, Jacob M. ; Shi, Yu Guang ; Lynch, Christopher J. ; Wang, Hong-Gang. / Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. In: Scientific reports. 2016 ; Vol. 6.
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Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. / Liu, Ying; Takahashi, Yoshinori; Desai, Neelam; Zhang, Jun; Serfass, Jacob M.; Shi, Yu Guang; Lynch, Christopher J.; Wang, Hong-Gang.

In: Scientific reports, Vol. 6, 20453, 09.02.2016.

Research output: Contribution to journalArticle

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