Biliary bile acid profiles of domestic fowl as determined by high performance liquid chromatography and fast atom bombardment mass spectrometry

R. G. Elkin, K. V. Wood, L. R. Hagey

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

1. 1. The biliary bile acid profiles of domestic chickens (Gallus domesticus), turkeys (Meleagris gallopavo), and ducks (Anas platyrhynchos) were determiend by high performance liquid chromatography (HPLC) and fast atom bombardment mass spectrometry (FAB-MS). 2. 2. Chenodeoxycholyltaurine and cholyltaurine were the predominant bile acids in chicken and turkey bile, whereas duck bile contained primarily chenodeoxycholyltaurine and phocaecholyltaurine. 3. 3. Allocholyltaurine was also detected in chicken and turkey bile, but not in duck bile. 4. 4. FAB-MS analyses of individual HPLC peak fractions from chicken and duck bile extracts confirmed the presence of either taurine-conjugated dihydroxy- or trihydroxycholanoates. 5. 5. Direct FAB-MS analyses of avian bile extracts not subjected to HPLC permitted a rapid assessment of the relative proportion of taurine-conjugated dihydroxy- to trihydroxycholanoates.

Original languageEnglish (US)
Pages (from-to)157-161
Number of pages5
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume96
Issue number1
DOIs
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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