Binary DNA arrays on heterogeneous patterned surfaces

Gang Zhang, Xin Yan, Xueliang Hou, Guang Lu, Bai Yang, Lixin Wu, Jiacong Shen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Studies of the deoxyribonucleic acid (DNA) molecule at surfaces are driven by both the inherent interest in understanding different aspects of this molecule and its importance in biomimetic materials science and molecular electronics. In this paper, we fabricate binary DNA/surfactant-modified DNA arrays on a heterogeneous patterned surface through twice controlled condensation and dewetting processes. Through the μCP technique, we created a hydrophobic/hydrophilic pattern, using a condensed microdroplet array as a template to fabricate a porous film of polystyrene (PS). With similarity to the "membrane-based patterning" method, this PS porous film was used as a mask, exposed hydrophilic regions inducing DNA to form as an array. After the PS porous films are peeled off, blocked surface sites emerged for the subsequent adsorption with other species. Through another condensation process, where a DNA aqueous solution droplet array could be used as a template, surfactant-modified DNA was sited in the ordered hydrophobic region through a second dewetting process and hydrophobic interaction, and we fabricated binary arrays on the same surface. At the same time, the double-helical structure and function of DNA still remained. This provides a simple, flexible, and efficient patterning technique for biomolecules.

Original languageEnglish (US)
Pages (from-to)9850-9854
Number of pages5
JournalLangmuir
Volume19
Issue number23
DOIs
StatePublished - Nov 11 2003

Fingerprint

DNA
deoxyribonucleic acid
Polystyrenes
polystyrene
Surface-Active Agents
drying
Condensation
Surface active agents
templates
condensation
Biomimetic materials
surfactants
Molecular electronics
Molecules
molecular electronics
biomimetics
Biomolecules
Materials science
materials science
Masks

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Zhang, G., Yan, X., Hou, X., Lu, G., Yang, B., Wu, L., & Shen, J. (2003). Binary DNA arrays on heterogeneous patterned surfaces. Langmuir, 19(23), 9850-9854. https://doi.org/10.1021/la035084e
Zhang, Gang ; Yan, Xin ; Hou, Xueliang ; Lu, Guang ; Yang, Bai ; Wu, Lixin ; Shen, Jiacong. / Binary DNA arrays on heterogeneous patterned surfaces. In: Langmuir. 2003 ; Vol. 19, No. 23. pp. 9850-9854.
@article{d824badae1a5463cacca34df257eff04,
title = "Binary DNA arrays on heterogeneous patterned surfaces",
abstract = "Studies of the deoxyribonucleic acid (DNA) molecule at surfaces are driven by both the inherent interest in understanding different aspects of this molecule and its importance in biomimetic materials science and molecular electronics. In this paper, we fabricate binary DNA/surfactant-modified DNA arrays on a heterogeneous patterned surface through twice controlled condensation and dewetting processes. Through the μCP technique, we created a hydrophobic/hydrophilic pattern, using a condensed microdroplet array as a template to fabricate a porous film of polystyrene (PS). With similarity to the {"}membrane-based patterning{"} method, this PS porous film was used as a mask, exposed hydrophilic regions inducing DNA to form as an array. After the PS porous films are peeled off, blocked surface sites emerged for the subsequent adsorption with other species. Through another condensation process, where a DNA aqueous solution droplet array could be used as a template, surfactant-modified DNA was sited in the ordered hydrophobic region through a second dewetting process and hydrophobic interaction, and we fabricated binary arrays on the same surface. At the same time, the double-helical structure and function of DNA still remained. This provides a simple, flexible, and efficient patterning technique for biomolecules.",
author = "Gang Zhang and Xin Yan and Xueliang Hou and Guang Lu and Bai Yang and Lixin Wu and Jiacong Shen",
year = "2003",
month = "11",
day = "11",
doi = "10.1021/la035084e",
language = "English (US)",
volume = "19",
pages = "9850--9854",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "23",

}

Zhang, G, Yan, X, Hou, X, Lu, G, Yang, B, Wu, L & Shen, J 2003, 'Binary DNA arrays on heterogeneous patterned surfaces', Langmuir, vol. 19, no. 23, pp. 9850-9854. https://doi.org/10.1021/la035084e

Binary DNA arrays on heterogeneous patterned surfaces. / Zhang, Gang; Yan, Xin; Hou, Xueliang; Lu, Guang; Yang, Bai; Wu, Lixin; Shen, Jiacong.

In: Langmuir, Vol. 19, No. 23, 11.11.2003, p. 9850-9854.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Binary DNA arrays on heterogeneous patterned surfaces

AU - Zhang, Gang

AU - Yan, Xin

AU - Hou, Xueliang

AU - Lu, Guang

AU - Yang, Bai

AU - Wu, Lixin

AU - Shen, Jiacong

PY - 2003/11/11

Y1 - 2003/11/11

N2 - Studies of the deoxyribonucleic acid (DNA) molecule at surfaces are driven by both the inherent interest in understanding different aspects of this molecule and its importance in biomimetic materials science and molecular electronics. In this paper, we fabricate binary DNA/surfactant-modified DNA arrays on a heterogeneous patterned surface through twice controlled condensation and dewetting processes. Through the μCP technique, we created a hydrophobic/hydrophilic pattern, using a condensed microdroplet array as a template to fabricate a porous film of polystyrene (PS). With similarity to the "membrane-based patterning" method, this PS porous film was used as a mask, exposed hydrophilic regions inducing DNA to form as an array. After the PS porous films are peeled off, blocked surface sites emerged for the subsequent adsorption with other species. Through another condensation process, where a DNA aqueous solution droplet array could be used as a template, surfactant-modified DNA was sited in the ordered hydrophobic region through a second dewetting process and hydrophobic interaction, and we fabricated binary arrays on the same surface. At the same time, the double-helical structure and function of DNA still remained. This provides a simple, flexible, and efficient patterning technique for biomolecules.

AB - Studies of the deoxyribonucleic acid (DNA) molecule at surfaces are driven by both the inherent interest in understanding different aspects of this molecule and its importance in biomimetic materials science and molecular electronics. In this paper, we fabricate binary DNA/surfactant-modified DNA arrays on a heterogeneous patterned surface through twice controlled condensation and dewetting processes. Through the μCP technique, we created a hydrophobic/hydrophilic pattern, using a condensed microdroplet array as a template to fabricate a porous film of polystyrene (PS). With similarity to the "membrane-based patterning" method, this PS porous film was used as a mask, exposed hydrophilic regions inducing DNA to form as an array. After the PS porous films are peeled off, blocked surface sites emerged for the subsequent adsorption with other species. Through another condensation process, where a DNA aqueous solution droplet array could be used as a template, surfactant-modified DNA was sited in the ordered hydrophobic region through a second dewetting process and hydrophobic interaction, and we fabricated binary arrays on the same surface. At the same time, the double-helical structure and function of DNA still remained. This provides a simple, flexible, and efficient patterning technique for biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=0345447096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345447096&partnerID=8YFLogxK

U2 - 10.1021/la035084e

DO - 10.1021/la035084e

M3 - Article

AN - SCOPUS:0345447096

VL - 19

SP - 9850

EP - 9854

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 23

ER -

Zhang G, Yan X, Hou X, Lu G, Yang B, Wu L et al. Binary DNA arrays on heterogeneous patterned surfaces. Langmuir. 2003 Nov 11;19(23):9850-9854. https://doi.org/10.1021/la035084e