Binding affinity of Plasmodium falciparum-infected erythrocytes from infected placentas and laboratory selected strains to chondroitin 4-sulfate

Rajeshwara N. Achur, Arivalagan Muthusamy, Subba Rao V. Madhunapantula, D. Channe Gowda

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The adherence of Plasmodium falciparum-infected red blood cells (IRBCs) in human placenta is mediated by chondroitin 4-sulfate (C4S). The C4S-adherent parasites selected from laboratory strains have been widely used for determining the C4S structural elements involved in IRBC binding and for the identification of parasite adhesive protein(s). However, as far as we know, the relative binding strength of the placental versus laboratory-selected parasites has not been reported. In this study, we show that IRBCs from the infected placentas bind to C4S about 3-fold higher than those selected for C4S adherence from laboratory strains. Although adherent parasites selected from several laboratory strains have comparable binding strengths, the one obtained from 3D7 parasites designated as 3D7N61 used for malaria genome sequencing, exhibits markedly lower binding strength. Furthermore, 3D7N61-CSA parasites lose most of the binding capacity by tenth generation in continuous culture.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalMolecular and biochemical parasitology
Volume159
Issue number1
DOIs
StatePublished - May 2008

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

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