Binding and neutralization characteristics of a panel of monoclonal antibodies to human papillomavirus 58

Sarah A. Brendle, Timothy D. Culp, Tatevik R. Broutian, Neil D. Christensen

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Human papillomavirus (HPV) 58 is a high-risk HPV type associated with progression to invasive genital carcinomas. We developed six monoclonal antibodies (mAbs) against HPV58 L1 virus-like particles that bind conformational epitopes on HPV58. The hybridoma cell lines were adapted to serum- and animal component-free conditions and the mAb supernatants were affinity-purified. The six mAbs neutralized HPV58 pseudoviruses (PsVs) and 'quasivirions' with different capacities. The mAbs differed in their ability to prevent PsV58 attachment to HaCaT cells, to the extracellular matrix (ECM) deposited by HaCaT cells, to heparin and to purified human laminin 5, a protein in the ECM. These mAbs provide a unique set of tools to study the binding properties of a previously untested, high-risk HPV type and the opportunity to compare these characteristics with the binding of other HPV types.

Original languageEnglish (US)
Pages (from-to)1834-1839
Number of pages6
JournalJournal of General Virology
Volume91
Issue number7
DOIs
StatePublished - Jul 2010

All Science Journal Classification (ASJC) codes

  • Virology

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