Binding of Caffeine and Quinine by Whey Protein and the Effect on Bitterness

Kelsey Tenney, John Hayes, Stephen Euston, Ryan Elias, John Coupland

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Many drugs and phytochemicals are bitter, leading to noncompliance with prescriptions and avoidance of healthy foods and a need to suppress their taste. The goal of this study was to investigate the binding of bitterants (quinine and caffeine) by whey protein isolate (WPI) and the effect on perceived bitterness. Caffeine interacted minimally with WPI, while the proportion of unbound quinine decreased exponentially with protein concentration. Molecular modeling was used to show the energy of the quinine-Β-lactoglubulin interaction was an order of magnitude greater than the caffeine-Β-lactoglobulin interaction. Untrained assessors were used to assess the bitterness of caffeine (1.8, 5.7, and 18 mM) and quinine (0.056, 0.10, and 0.18 mM) solutions with 0% or 1% WPI. There was no significant effect of protein on the bitterness of caffeine solutions, but WPI decreased the bitterness of quinine relative to the same concentration in water. This is generally consistent with our hypothesis that higher binding results in lower bitterness; however the magnitude of reduction was not large and the bitterness of the protein-quinine solutions was greater than would be expected for the unbound quinine present.

Original languageEnglish (US)
Pages (from-to)509-516
Number of pages8
JournalJournal of Food Science
Volume82
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

quinine
Quinine
bitterness
caffeine
Caffeine
whey protein
whey protein isolate
lactoglobulins
Lactoglobulins
Proteins
proteins
Phytochemicals
Whey Proteins
phytopharmaceuticals
Prescriptions
Food
drugs
Water
energy
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

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Binding of Caffeine and Quinine by Whey Protein and the Effect on Bitterness. / Tenney, Kelsey; Hayes, John; Euston, Stephen; Elias, Ryan; Coupland, John.

In: Journal of Food Science, Vol. 82, No. 2, 01.02.2017, p. 509-516.

Research output: Contribution to journalArticle

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