Binding of signal recognition particle give ribosomes/nascent chain complexes a competitive advantages in endoplasmic reticulum membrane interaction

Andrea Neuhof, Melissa Rolls, Berit Jungnickel, Kai Uwe Kalies, Tom A. Rapoport

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Most secretory and membrane proteins are sorted by signal sequences to the endoplasmic reticulum (ER) membrane early during their synthesis. Targeting of the ribosome-nascent chain complex (RNC) involves the binding of the signal sequence to the signal recognition particle (SRP), followed by an interaction of ribosomes-bound SRP with the SRP receptor. However, ribosomes can also independently bind to the ER translocation channel formed by the Sec61p complex. To explain the specificity of membrane targetting, it has therefore been proposed that nascent polypeptide-associated complex functions as a cytosolic inhibitor of signal sequence- and SRP -independent ribosomes binding to the ER membrane. We report here that SRP -independent binding of RNCs to the ER membrane can occur in the presence of all cytosolic factors, including nascent polypeptide-associated complex. Nontranslating ribosomes competitively inhibit SRP -independent membrane binding of RNCs but have no effect when SRP is bound to the RNCs. The protective effect of SRP against ribosome competition depends on a functional signal sequence in the nascent chain and is also observed with reconstituted proteoliposomes containing only the Sec61p complex and the SRP receptor. We conclude that cytosolic factors do not prevent the membrane binding of ribosomes. Instead, specific ribosomes targetting to the Sec61p complex is provided by the binding of SRP to RNCs followed by an interaction with SRP receptor, which gives RNC-SRP complexes a selective advantage in membrane targetting over nontranslating ribosomes.

Original languageEnglish (US)
Pages (from-to)103-115
Number of pages13
JournalMolecular biology of the cell
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1998

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Signal Recognition Particle
Ribosomes
Endoplasmic Reticulum
Membranes
Protein Sorting Signals
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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title = "Binding of signal recognition particle give ribosomes/nascent chain complexes a competitive advantages in endoplasmic reticulum membrane interaction",
abstract = "Most secretory and membrane proteins are sorted by signal sequences to the endoplasmic reticulum (ER) membrane early during their synthesis. Targeting of the ribosome-nascent chain complex (RNC) involves the binding of the signal sequence to the signal recognition particle (SRP), followed by an interaction of ribosomes-bound SRP with the SRP receptor. However, ribosomes can also independently bind to the ER translocation channel formed by the Sec61p complex. To explain the specificity of membrane targetting, it has therefore been proposed that nascent polypeptide-associated complex functions as a cytosolic inhibitor of signal sequence- and SRP -independent ribosomes binding to the ER membrane. We report here that SRP -independent binding of RNCs to the ER membrane can occur in the presence of all cytosolic factors, including nascent polypeptide-associated complex. Nontranslating ribosomes competitively inhibit SRP -independent membrane binding of RNCs but have no effect when SRP is bound to the RNCs. The protective effect of SRP against ribosome competition depends on a functional signal sequence in the nascent chain and is also observed with reconstituted proteoliposomes containing only the Sec61p complex and the SRP receptor. We conclude that cytosolic factors do not prevent the membrane binding of ribosomes. Instead, specific ribosomes targetting to the Sec61p complex is provided by the binding of SRP to RNCs followed by an interaction with SRP receptor, which gives RNC-SRP complexes a selective advantage in membrane targetting over nontranslating ribosomes.",
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Binding of signal recognition particle give ribosomes/nascent chain complexes a competitive advantages in endoplasmic reticulum membrane interaction. / Neuhof, Andrea; Rolls, Melissa; Jungnickel, Berit; Kalies, Kai Uwe; Rapoport, Tom A.

In: Molecular biology of the cell, Vol. 9, No. 1, 01.01.1998, p. 103-115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Binding of signal recognition particle give ribosomes/nascent chain complexes a competitive advantages in endoplasmic reticulum membrane interaction

AU - Neuhof, Andrea

AU - Rolls, Melissa

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AU - Kalies, Kai Uwe

AU - Rapoport, Tom A.

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