Bio-organism sensing via surface enhanced Raman spectroscopy on controlled metal/polymer nanostructured substrates

M. C. Demirel, S. Kao, N. Malvadkar, H. Wang, X. Gong, M. Poss, D. L. Allara

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A new class of nonlithographically prepared surface enhanced Raman spectroscopy SERS substrates based on metalized, nanostructured poly p-xylylene films has been developed and optimized for surface plasmon response with a view to applications of SERS detection of microbial pathogens, specifically, bacteria and viruses. The main emphasis has been on achieving high spot to spot, sample to sample reproducibility of the SERS signals while maintaining useful enhancement factors. The use of these surfaces, metalized with either Ag or Au, provides a noninvasive and nondestructive method for spectral fingerprint analyses of both bacteria and viruses. Examples are given for the detection of bacteria E. coli and B. cereus and viruses respiratory syncytial virus and Coxsackievirus. Our method is able to distinguish Gram positive from Gram negative bacterial strains as well as enveloped and nonenveloped viruses. The results demonstrate the development of a new class of SERS substrates which can provide rapid, selective identification of infectious agents without amplification of cultures.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalBiointerphases
Volume4
Issue number2
DOIs
StatePublished - Jun 2009

Fingerprint

Raman Spectrum Analysis
viruses
Viruses
organisms
Raman spectroscopy
Polymers
Metals
Bacteria
bacteria
polymers
Substrates
metals
Cercopithecine Herpesvirus 1
Respiratory Syncytial Viruses
Enterovirus
Dermatoglyphics
pathogens
Escherichia coli
Pathogens
Amplification

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Demirel, M. C. ; Kao, S. ; Malvadkar, N. ; Wang, H. ; Gong, X. ; Poss, M. ; Allara, D. L. / Bio-organism sensing via surface enhanced Raman spectroscopy on controlled metal/polymer nanostructured substrates. In: Biointerphases. 2009 ; Vol. 4, No. 2. pp. 35-41.
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Bio-organism sensing via surface enhanced Raman spectroscopy on controlled metal/polymer nanostructured substrates. / Demirel, M. C.; Kao, S.; Malvadkar, N.; Wang, H.; Gong, X.; Poss, M.; Allara, D. L.

In: Biointerphases, Vol. 4, No. 2, 06.2009, p. 35-41.

Research output: Contribution to journalArticle

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