Bioactive growth hormone in older men and women

It's relationship to immune markers and healthspan

William J. Kraemer, Mary J. Kennett, Andrea Marie Mastro, Roger J. McCarter, Connie Jo Rogers, William H. DuPont, Shawn D. Flanagan, William J. Turbitt, Maren S. Fragala, Emily M. Post, Wesley C. Hymer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective The consequences of age-related decline in the somatotropic axis of humans are complex and remain largely unresolved. We tested the hypothesis that hGH measurements of plasma by bioassay vs immunoassay from samples obtained from free-living, elderly individuals would reveal a dichotomy in GH activities that are correlated with the functional status of the donors, i.e. their healthspan. Design Forty-one men and women of advanced age (men: N = 16, age, 80.5 ± 6.5 years; height, 173.1 ± 6.9 cm; body mass, 81.8 ± 13.0 kg) and (women: N = 25, age, 80.7 ± 7.2 years; height, 157.7 ± 6.0 cm; body mass, 68.8 ± 17 kg), were recruited for a cross-sectional study. Participants filled out PROMIS (Patient-Reported Outcomes Measurement Information System, U. S. Department of Health and Human Services) scales, undertook physical performance tests and had fasted blood samples obtained at rest for measurement of hormonal and immunology biomarkers. Results When measured by the well-established rat tibial line GH bioassay, one half of the plasma samples (n = 20) contained bioassayable GH (bGH), but the other half (n = 21) failed to mount increases in tibial plate width above saline injected controls. This difference did not correlate with the age, sex or physical functionality of the plasma donor. It also did not correlate with hGH concentrations measured by immunoassay. In those cases in which bGH was detected, various hierarchical regression models predicted that GHRH, c-peptide, VEGF, NPY, IL-4 and T-regulatory lymphocytes were associated with the difference and predicted bGH. Conclusion Results from this study suggest that the actions of bGH at the cellular level may be modified by other factors and that this may explain the lack of correlations observed in this study.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalGrowth Hormone and IGF Research
Volume34
DOIs
StatePublished - Jun 1 2017

Fingerprint

Growth Hormone
Biomarkers
Immunoassay
Biological Assay
Tissue Donors
United States Dept. of Health and Human Services
Regulatory T-Lymphocytes
Allergy and Immunology
Information Systems
Interleukin-4
Vascular Endothelial Growth Factor A
Cross-Sectional Studies
Peptides
Patient Reported Outcome Measures

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Kraemer, William J. ; Kennett, Mary J. ; Mastro, Andrea Marie ; McCarter, Roger J. ; Rogers, Connie Jo ; DuPont, William H. ; Flanagan, Shawn D. ; Turbitt, William J. ; Fragala, Maren S. ; Post, Emily M. ; Hymer, Wesley C. / Bioactive growth hormone in older men and women : It's relationship to immune markers and healthspan. In: Growth Hormone and IGF Research. 2017 ; Vol. 34. pp. 45-54.
@article{da0994d12be64320b3d0add14d08c291,
title = "Bioactive growth hormone in older men and women: It's relationship to immune markers and healthspan",
abstract = "Objective The consequences of age-related decline in the somatotropic axis of humans are complex and remain largely unresolved. We tested the hypothesis that hGH measurements of plasma by bioassay vs immunoassay from samples obtained from free-living, elderly individuals would reveal a dichotomy in GH activities that are correlated with the functional status of the donors, i.e. their healthspan. Design Forty-one men and women of advanced age (men: N = 16, age, 80.5 ± 6.5 years; height, 173.1 ± 6.9 cm; body mass, 81.8 ± 13.0 kg) and (women: N = 25, age, 80.7 ± 7.2 years; height, 157.7 ± 6.0 cm; body mass, 68.8 ± 17 kg), were recruited for a cross-sectional study. Participants filled out PROMIS (Patient-Reported Outcomes Measurement Information System, U. S. Department of Health and Human Services) scales, undertook physical performance tests and had fasted blood samples obtained at rest for measurement of hormonal and immunology biomarkers. Results When measured by the well-established rat tibial line GH bioassay, one half of the plasma samples (n = 20) contained bioassayable GH (bGH), but the other half (n = 21) failed to mount increases in tibial plate width above saline injected controls. This difference did not correlate with the age, sex or physical functionality of the plasma donor. It also did not correlate with hGH concentrations measured by immunoassay. In those cases in which bGH was detected, various hierarchical regression models predicted that GHRH, c-peptide, VEGF, NPY, IL-4 and T-regulatory lymphocytes were associated with the difference and predicted bGH. Conclusion Results from this study suggest that the actions of bGH at the cellular level may be modified by other factors and that this may explain the lack of correlations observed in this study.",
author = "Kraemer, {William J.} and Kennett, {Mary J.} and Mastro, {Andrea Marie} and McCarter, {Roger J.} and Rogers, {Connie Jo} and DuPont, {William H.} and Flanagan, {Shawn D.} and Turbitt, {William J.} and Fragala, {Maren S.} and Post, {Emily M.} and Hymer, {Wesley C.}",
year = "2017",
month = "6",
day = "1",
doi = "10.1016/j.ghir.2017.05.002",
language = "English (US)",
volume = "34",
pages = "45--54",
journal = "Growth Hormone and IGF Research",
issn = "1096-6374",

}

Kraemer, WJ, Kennett, MJ, Mastro, AM, McCarter, RJ, Rogers, CJ, DuPont, WH, Flanagan, SD, Turbitt, WJ, Fragala, MS, Post, EM & Hymer, WC 2017, 'Bioactive growth hormone in older men and women: It's relationship to immune markers and healthspan', Growth Hormone and IGF Research, vol. 34, pp. 45-54. https://doi.org/10.1016/j.ghir.2017.05.002

Bioactive growth hormone in older men and women : It's relationship to immune markers and healthspan. / Kraemer, William J.; Kennett, Mary J.; Mastro, Andrea Marie; McCarter, Roger J.; Rogers, Connie Jo; DuPont, William H.; Flanagan, Shawn D.; Turbitt, William J.; Fragala, Maren S.; Post, Emily M.; Hymer, Wesley C.

In: Growth Hormone and IGF Research, Vol. 34, 01.06.2017, p. 45-54.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioactive growth hormone in older men and women

T2 - It's relationship to immune markers and healthspan

AU - Kraemer, William J.

AU - Kennett, Mary J.

AU - Mastro, Andrea Marie

AU - McCarter, Roger J.

AU - Rogers, Connie Jo

AU - DuPont, William H.

AU - Flanagan, Shawn D.

AU - Turbitt, William J.

AU - Fragala, Maren S.

AU - Post, Emily M.

AU - Hymer, Wesley C.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Objective The consequences of age-related decline in the somatotropic axis of humans are complex and remain largely unresolved. We tested the hypothesis that hGH measurements of plasma by bioassay vs immunoassay from samples obtained from free-living, elderly individuals would reveal a dichotomy in GH activities that are correlated with the functional status of the donors, i.e. their healthspan. Design Forty-one men and women of advanced age (men: N = 16, age, 80.5 ± 6.5 years; height, 173.1 ± 6.9 cm; body mass, 81.8 ± 13.0 kg) and (women: N = 25, age, 80.7 ± 7.2 years; height, 157.7 ± 6.0 cm; body mass, 68.8 ± 17 kg), were recruited for a cross-sectional study. Participants filled out PROMIS (Patient-Reported Outcomes Measurement Information System, U. S. Department of Health and Human Services) scales, undertook physical performance tests and had fasted blood samples obtained at rest for measurement of hormonal and immunology biomarkers. Results When measured by the well-established rat tibial line GH bioassay, one half of the plasma samples (n = 20) contained bioassayable GH (bGH), but the other half (n = 21) failed to mount increases in tibial plate width above saline injected controls. This difference did not correlate with the age, sex or physical functionality of the plasma donor. It also did not correlate with hGH concentrations measured by immunoassay. In those cases in which bGH was detected, various hierarchical regression models predicted that GHRH, c-peptide, VEGF, NPY, IL-4 and T-regulatory lymphocytes were associated with the difference and predicted bGH. Conclusion Results from this study suggest that the actions of bGH at the cellular level may be modified by other factors and that this may explain the lack of correlations observed in this study.

AB - Objective The consequences of age-related decline in the somatotropic axis of humans are complex and remain largely unresolved. We tested the hypothesis that hGH measurements of plasma by bioassay vs immunoassay from samples obtained from free-living, elderly individuals would reveal a dichotomy in GH activities that are correlated with the functional status of the donors, i.e. their healthspan. Design Forty-one men and women of advanced age (men: N = 16, age, 80.5 ± 6.5 years; height, 173.1 ± 6.9 cm; body mass, 81.8 ± 13.0 kg) and (women: N = 25, age, 80.7 ± 7.2 years; height, 157.7 ± 6.0 cm; body mass, 68.8 ± 17 kg), were recruited for a cross-sectional study. Participants filled out PROMIS (Patient-Reported Outcomes Measurement Information System, U. S. Department of Health and Human Services) scales, undertook physical performance tests and had fasted blood samples obtained at rest for measurement of hormonal and immunology biomarkers. Results When measured by the well-established rat tibial line GH bioassay, one half of the plasma samples (n = 20) contained bioassayable GH (bGH), but the other half (n = 21) failed to mount increases in tibial plate width above saline injected controls. This difference did not correlate with the age, sex or physical functionality of the plasma donor. It also did not correlate with hGH concentrations measured by immunoassay. In those cases in which bGH was detected, various hierarchical regression models predicted that GHRH, c-peptide, VEGF, NPY, IL-4 and T-regulatory lymphocytes were associated with the difference and predicted bGH. Conclusion Results from this study suggest that the actions of bGH at the cellular level may be modified by other factors and that this may explain the lack of correlations observed in this study.

UR - http://www.scopus.com/inward/record.url?scp=85019925316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019925316&partnerID=8YFLogxK

U2 - 10.1016/j.ghir.2017.05.002

DO - 10.1016/j.ghir.2017.05.002

M3 - Article

VL - 34

SP - 45

EP - 54

JO - Growth Hormone and IGF Research

JF - Growth Hormone and IGF Research

SN - 1096-6374

ER -