Biochemical and morphological study of adriamycin-induced changes in murine neuroblastoma cells

Cara-Lynne Schengrund, B. A. Sheffler

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cholinergic murine neuroblastoma cells, maintained in vitro, were exposed to a low concentration (0.4 μg/ml) of adriamycin. Morphologically the treated cells appeared to differentiate. The cell bodies increased in size from an average fixed cell body diameter of 7-13 to 25-40 μm, the cells developed long processes, became argyrophilic and the percentage of cells undergoing mitosis decreased relative to controls. Acetylcholine esterase activity increased in the drug-treated cells suggesting induction of differentiation. However, choline acetyltransferase activity and ganglioside composition remained unchanged. In addition, inoculation of mice with 2 x 105 viable drug-treated or control cells resulted in all of the mice developing neuroblastoma. No differences were observed in either the rate of tumor development or survial times. These results suggest that neuroblastoma cells may survive adriamycin treatment by becoming 'differentiated', ceasing cell division until conditions favor their undergoing another cell cycle.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalOncology
Volume39
Issue number3
DOIs
StatePublished - Jan 1 1982

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Neuroblastoma
Doxorubicin
Choline O-Acetyltransferase
Gangliosides
Esterases
Mitosis
Pharmaceutical Preparations
Cell Division
Cholinergic Agents
Acetylcholine
Cell Cycle
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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abstract = "Cholinergic murine neuroblastoma cells, maintained in vitro, were exposed to a low concentration (0.4 μg/ml) of adriamycin. Morphologically the treated cells appeared to differentiate. The cell bodies increased in size from an average fixed cell body diameter of 7-13 to 25-40 μm, the cells developed long processes, became argyrophilic and the percentage of cells undergoing mitosis decreased relative to controls. Acetylcholine esterase activity increased in the drug-treated cells suggesting induction of differentiation. However, choline acetyltransferase activity and ganglioside composition remained unchanged. In addition, inoculation of mice with 2 x 105 viable drug-treated or control cells resulted in all of the mice developing neuroblastoma. No differences were observed in either the rate of tumor development or survial times. These results suggest that neuroblastoma cells may survive adriamycin treatment by becoming 'differentiated', ceasing cell division until conditions favor their undergoing another cell cycle.",
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Biochemical and morphological study of adriamycin-induced changes in murine neuroblastoma cells. / Schengrund, Cara-Lynne; Sheffler, B. A.

In: Oncology, Vol. 39, No. 3, 01.01.1982, p. 185-190.

Research output: Contribution to journalArticle

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