Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli

Alice S. Pereira, Pedro Tavares, Carsten Krebs, Boi Hanh Huynh, Frank Rusnak, Isabel Moura, José J.G. Moura

Research output: Contribution to journalArticle

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Abstract

Fuscoredoxin is a unique iron containing protein of yet unknown function originally discovered in the sulfate reducers of the genus Desulfovibrio. It contains two iron-sulfur clusters: a cubane [4Fe-4S] and a unprecedented structure. The recent determination of the genomic sequence of Escherichia coli (E. coli) has revealed a homologue of fuscoredoxin in this facultative microbe. The presence of this gene in E. coli raises interesting questions regarding the function of fuscoredoxin and whether this gene represents a structural homologue of the better-characterized Desulfovibrio proteins. In order to explore the latter, an overexpression system for the E. coli fuscoredoxin gene was devised. The gene was cloned from genomic DNA by use of the polymerase chain reaction into the expression vector pT7-7 and overexpressed in E. coli BL21(DE3) cells. After two chromatographic steps a good yield of recombinant protein was obtained (approximately 4 mg of pure protein per liter of culture). The purified protein exhibits an optical spectrum characteristic of the homologue from D. desulfuricans, indicating that cofactor assembly was accomplished. Iron analysis indicated that the protein contains circa 8 iron atoms/molecule which were shown by EPR and Mossbauer spectroscopies to be present as two multinuclear clusters, albeit with slightly altered spectroscopic features. A comparison of the primary sequences of fuscoredoxins is presented and differences on cluster coordination modes are discussed on the light of the spectroscopic data.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume260
Issue number1
DOIs
StatePublished - Jun 24 1999

Fingerprint

Escherichia coli
Iron
Genes
Desulfovibrio
Proteins
Iron analysis
Mossbauer Spectroscopy
Polymerase chain reaction
Mossbauer spectroscopy
DNA-Directed DNA Polymerase
Sulfur
Recombinant Proteins
Sulfates
Paramagnetic resonance
Sequence Analysis
Desulfovibrio desulfuricans fuscoredoxin protein
Light
Polymerase Chain Reaction
Atoms
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Pereira, Alice S. ; Tavares, Pedro ; Krebs, Carsten ; Huynh, Boi Hanh ; Rusnak, Frank ; Moura, Isabel ; Moura, José J.G. / Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 260, No. 1. pp. 209-215.
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Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli. / Pereira, Alice S.; Tavares, Pedro; Krebs, Carsten; Huynh, Boi Hanh; Rusnak, Frank; Moura, Isabel; Moura, José J.G.

In: Biochemical and Biophysical Research Communications, Vol. 260, No. 1, 24.06.1999, p. 209-215.

Research output: Contribution to journalArticle

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