Biochemical localization of a protein involved in synthesis of Gluconacetobacter hansenii cellulose

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Abstract

Using subcellular fractionation and Western blot methods, we have shown that AcsD, one of the proteins encoded by the Acetobacter cellulose synthase (acs) operon, is localized in the periplasmic region of the cell. AcsD protein was heterologously expressed in Escherichia coli and purified using histidine tag affinity methods. The purified protein was used to obtain rabbit polyclonal antibodies. The purity of the subcellular fractions was assessed by marker enzyme assays.

Original languageEnglish (US)
Pages (from-to)739-747
Number of pages9
JournalCellulose
Volume18
Issue number3
DOIs
StatePublished - Jun 1 2011

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

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