Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain

Nazlina Haiza Mohd Yasin, Masaharu Fukuzaki, Toshinari Maeda, Toshiki Miyazaki, Che Mohd Hakiman Che Maail, Hidayah Ariffin, Thomas K. Wood

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm frond (OPF) juice and sewage sludge as substrates. Substrate improvement was accomplished when hydrogen productivity increased 8-fold after enzymatic treatment of the sludge with a mixture of amylase and cellulase. The OPF juice with sewage sludge provided an optimum carbon/nitrogen ratio since the yield of biohydrogen increased to 1.5 from 1.3 mol H2/mol glucose compared to our previous study. In this study, we also reveal that our engineered strain improved 200-fold biohydrogen productivity from biomass sources compared to the unmodified host. In conclusion, we determined that our engineered strain can use biomass as an alternative substrate for enhanced biohydrogen production.

Original languageEnglish (US)
Pages (from-to)10277-10283
Number of pages7
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number25
DOIs
StatePublished - Aug 21 2013

Fingerprint

juices
sewage
sludge
Palm oil
Sewage sludge
biomass
Escherichia
Escherichia coli
Biomass
oils
productivity
glucose
Glucose
Substrates
Productivity
Amylases
energy sources
Nitrogen
nitrogen
Hydrogen

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Yasin, Nazlina Haiza Mohd ; Fukuzaki, Masaharu ; Maeda, Toshinari ; Miyazaki, Toshiki ; Maail, Che Mohd Hakiman Che ; Ariffin, Hidayah ; Wood, Thomas K. / Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain. In: International Journal of Hydrogen Energy. 2013 ; Vol. 38, No. 25. pp. 10277-10283.
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Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain. / Yasin, Nazlina Haiza Mohd; Fukuzaki, Masaharu; Maeda, Toshinari; Miyazaki, Toshiki; Maail, Che Mohd Hakiman Che; Ariffin, Hidayah; Wood, Thomas K.

In: International Journal of Hydrogen Energy, Vol. 38, No. 25, 21.08.2013, p. 10277-10283.

Research output: Contribution to journalArticle

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AU - Maail, Che Mohd Hakiman Che

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