Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes: A study of test-retest reliability

Sheila Grace West, P. Wagner, S. L. Schoemer, K. D. Hecker, K. L. Hurston, A. Likos Krick, L. Boseska, Jan Ulbrecht, A. L. Hinderliter

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Aims/hypothesis. Dysfunction of the vascular endothelium is commonly observed in Type 2 diabetes, and endothelial function may be an important outcome for clinical trials in diabetic samples. However, the most commonly used non-invasive test of endothelial function (flow-mediated dilation [FMD]) is technically challenging to perform, and no previous studies have carefully examined the reproducibility of FMD measurements in individuals with Type 2 diabetes. In this study, we tested the hypothesis that larger day-to-day changes in insulin and glucose are associated with larger fluctuations in FMD. Methods. Ultrasound was used to measure the FMD (% change from baseline diameter) of the brachial artery in 18 healthy adults with Type 2 diabetes on three separate occasions, in the absence of changes to diet, activity level or medications. The CV and mean deviations between pairs of FMD scores in the same individual were used as the primary outcome variables. Results. The CV for FMD (29.7%) was higher than the level traditionally accepted for biochemical assays. However, this CV estimate is within the low range of published values for FMD in healthy individuals. FMD scores were not significantly correlated with glucose or insulin levels. However, subjects with the largest variability in FMD also showed the largest fluctuations in glucose (r=0.52), insulin (r=0.47) and heart rate (r=0.48) (p≤0.05). Conclusions/interpretation. FMD can be reliably measured in individuals with Type 2 diabetes, and population-specific data on reliability is critical for the design of adequately powered studies of endothelial function.

Original languageEnglish (US)
Pages (from-to)1625-1631
Number of pages7
JournalDiabetologia
Volume47
Issue number9
DOIs
StatePublished - Sep 1 2004

Fingerprint

Reproducibility of Results
Type 2 Diabetes Mellitus
Dilatation
Insulin
Glucose
Brachial Artery
Vascular Endothelium
Heart Rate
Clinical Trials
Diet

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

West, Sheila Grace ; Wagner, P. ; Schoemer, S. L. ; Hecker, K. D. ; Hurston, K. L. ; Krick, A. Likos ; Boseska, L. ; Ulbrecht, Jan ; Hinderliter, A. L. / Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes : A study of test-retest reliability. In: Diabetologia. 2004 ; Vol. 47, No. 9. pp. 1625-1631.
@article{13fa38d206354ee99dead1f99f7f7d13,
title = "Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes: A study of test-retest reliability",
abstract = "Aims/hypothesis. Dysfunction of the vascular endothelium is commonly observed in Type 2 diabetes, and endothelial function may be an important outcome for clinical trials in diabetic samples. However, the most commonly used non-invasive test of endothelial function (flow-mediated dilation [FMD]) is technically challenging to perform, and no previous studies have carefully examined the reproducibility of FMD measurements in individuals with Type 2 diabetes. In this study, we tested the hypothesis that larger day-to-day changes in insulin and glucose are associated with larger fluctuations in FMD. Methods. Ultrasound was used to measure the FMD ({\%} change from baseline diameter) of the brachial artery in 18 healthy adults with Type 2 diabetes on three separate occasions, in the absence of changes to diet, activity level or medications. The CV and mean deviations between pairs of FMD scores in the same individual were used as the primary outcome variables. Results. The CV for FMD (29.7{\%}) was higher than the level traditionally accepted for biochemical assays. However, this CV estimate is within the low range of published values for FMD in healthy individuals. FMD scores were not significantly correlated with glucose or insulin levels. However, subjects with the largest variability in FMD also showed the largest fluctuations in glucose (r=0.52), insulin (r=0.47) and heart rate (r=0.48) (p≤0.05). Conclusions/interpretation. FMD can be reliably measured in individuals with Type 2 diabetes, and population-specific data on reliability is critical for the design of adequately powered studies of endothelial function.",
author = "West, {Sheila Grace} and P. Wagner and Schoemer, {S. L.} and Hecker, {K. D.} and Hurston, {K. L.} and Krick, {A. Likos} and L. Boseska and Jan Ulbrecht and Hinderliter, {A. L.}",
year = "2004",
month = "9",
day = "1",
doi = "10.1007/s00125-004-1502-8",
language = "English (US)",
volume = "47",
pages = "1625--1631",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer Verlag",
number = "9",

}

West, SG, Wagner, P, Schoemer, SL, Hecker, KD, Hurston, KL, Krick, AL, Boseska, L, Ulbrecht, J & Hinderliter, AL 2004, 'Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes: A study of test-retest reliability', Diabetologia, vol. 47, no. 9, pp. 1625-1631. https://doi.org/10.1007/s00125-004-1502-8

Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes : A study of test-retest reliability. / West, Sheila Grace; Wagner, P.; Schoemer, S. L.; Hecker, K. D.; Hurston, K. L.; Krick, A. Likos; Boseska, L.; Ulbrecht, Jan; Hinderliter, A. L.

In: Diabetologia, Vol. 47, No. 9, 01.09.2004, p. 1625-1631.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biological correlates of day-to-day variation in flow-mediated dilation in individuals with Type 2 diabetes

T2 - A study of test-retest reliability

AU - West, Sheila Grace

AU - Wagner, P.

AU - Schoemer, S. L.

AU - Hecker, K. D.

AU - Hurston, K. L.

AU - Krick, A. Likos

AU - Boseska, L.

AU - Ulbrecht, Jan

AU - Hinderliter, A. L.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - Aims/hypothesis. Dysfunction of the vascular endothelium is commonly observed in Type 2 diabetes, and endothelial function may be an important outcome for clinical trials in diabetic samples. However, the most commonly used non-invasive test of endothelial function (flow-mediated dilation [FMD]) is technically challenging to perform, and no previous studies have carefully examined the reproducibility of FMD measurements in individuals with Type 2 diabetes. In this study, we tested the hypothesis that larger day-to-day changes in insulin and glucose are associated with larger fluctuations in FMD. Methods. Ultrasound was used to measure the FMD (% change from baseline diameter) of the brachial artery in 18 healthy adults with Type 2 diabetes on three separate occasions, in the absence of changes to diet, activity level or medications. The CV and mean deviations between pairs of FMD scores in the same individual were used as the primary outcome variables. Results. The CV for FMD (29.7%) was higher than the level traditionally accepted for biochemical assays. However, this CV estimate is within the low range of published values for FMD in healthy individuals. FMD scores were not significantly correlated with glucose or insulin levels. However, subjects with the largest variability in FMD also showed the largest fluctuations in glucose (r=0.52), insulin (r=0.47) and heart rate (r=0.48) (p≤0.05). Conclusions/interpretation. FMD can be reliably measured in individuals with Type 2 diabetes, and population-specific data on reliability is critical for the design of adequately powered studies of endothelial function.

AB - Aims/hypothesis. Dysfunction of the vascular endothelium is commonly observed in Type 2 diabetes, and endothelial function may be an important outcome for clinical trials in diabetic samples. However, the most commonly used non-invasive test of endothelial function (flow-mediated dilation [FMD]) is technically challenging to perform, and no previous studies have carefully examined the reproducibility of FMD measurements in individuals with Type 2 diabetes. In this study, we tested the hypothesis that larger day-to-day changes in insulin and glucose are associated with larger fluctuations in FMD. Methods. Ultrasound was used to measure the FMD (% change from baseline diameter) of the brachial artery in 18 healthy adults with Type 2 diabetes on three separate occasions, in the absence of changes to diet, activity level or medications. The CV and mean deviations between pairs of FMD scores in the same individual were used as the primary outcome variables. Results. The CV for FMD (29.7%) was higher than the level traditionally accepted for biochemical assays. However, this CV estimate is within the low range of published values for FMD in healthy individuals. FMD scores were not significantly correlated with glucose or insulin levels. However, subjects with the largest variability in FMD also showed the largest fluctuations in glucose (r=0.52), insulin (r=0.47) and heart rate (r=0.48) (p≤0.05). Conclusions/interpretation. FMD can be reliably measured in individuals with Type 2 diabetes, and population-specific data on reliability is critical for the design of adequately powered studies of endothelial function.

UR - http://www.scopus.com/inward/record.url?scp=5644272818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5644272818&partnerID=8YFLogxK

U2 - 10.1007/s00125-004-1502-8

DO - 10.1007/s00125-004-1502-8

M3 - Article

C2 - 15365618

AN - SCOPUS:5644272818

VL - 47

SP - 1625

EP - 1631

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 9

ER -