Biological hydrogen production measured in batch anaerobic respirometers

Bruce Ernest Logan, Sang Eun Oh, In S. Kim, Steven Van Ginkel

Research output: Contribution to journalArticle

417 Citations (Scopus)

Abstract

The biological production of hydrogen from the fermentation of different substrates was examined in batch tests using heat-shocked mixed cultures with two techniques: an intermittent pressure release method (Owen method) and a continuous gas release method using a bubble measurement device (respirometric method). Under otherwise identical conditions, the respirometric method resulted in the production of 43% more hydrogen gas from glucose than the Owen method. The lower conversion of glucose to hydrogen using the Owen protocol may have been produced by repression of hydrogenase activity from high partial pressures in the gastight bottles, but this could not be proven using a thermodynamic/rate inhibition analysis. In the respirometric method, total pressure in the headspace never exceeded ambient pressure, and hydrogen typically composed as much as 62% of the headspace gas. High conversion efficiencies were consistently obtained with heat-shocked soils taken at different times and those stored for up to a month. Hydrogen gas composition was consistently in the range of 60-64% for glucose-grown cultures during logarithmic growth but declined in stationary cultures. Overall, hydrogen conversion efficiencies for glucose cultures were 23% based on the assumption of a maximum of 4 mol of hydrogen/mol of glucose. Hydrogen conversion efficiencies were similar for sucrose (23%) and somewhat lower for molasses (15%) but were much lower for lactate (0.50%) and cellulose (0.075%).

Original languageEnglish (US)
Pages (from-to)2530-2535
Number of pages6
JournalEnvironmental Science and Technology
Volume36
Issue number11
DOIs
StatePublished - Jun 1 2002

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Hydrogen production
Hydrogen
hydrogen
glucose
Glucose
Gases
Conversion efficiency
gas
Molasses
Hydrogenase
biological production
Bottles
method
partial pressure
sucrose
Cellulose
Partial pressure
Fermentation
fermentation
Sucrose

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Logan, Bruce Ernest ; Oh, Sang Eun ; Kim, In S. ; Van Ginkel, Steven. / Biological hydrogen production measured in batch anaerobic respirometers. In: Environmental Science and Technology. 2002 ; Vol. 36, No. 11. pp. 2530-2535.
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abstract = "The biological production of hydrogen from the fermentation of different substrates was examined in batch tests using heat-shocked mixed cultures with two techniques: an intermittent pressure release method (Owen method) and a continuous gas release method using a bubble measurement device (respirometric method). Under otherwise identical conditions, the respirometric method resulted in the production of 43{\%} more hydrogen gas from glucose than the Owen method. The lower conversion of glucose to hydrogen using the Owen protocol may have been produced by repression of hydrogenase activity from high partial pressures in the gastight bottles, but this could not be proven using a thermodynamic/rate inhibition analysis. In the respirometric method, total pressure in the headspace never exceeded ambient pressure, and hydrogen typically composed as much as 62{\%} of the headspace gas. High conversion efficiencies were consistently obtained with heat-shocked soils taken at different times and those stored for up to a month. Hydrogen gas composition was consistently in the range of 60-64{\%} for glucose-grown cultures during logarithmic growth but declined in stationary cultures. Overall, hydrogen conversion efficiencies for glucose cultures were 23{\%} based on the assumption of a maximum of 4 mol of hydrogen/mol of glucose. Hydrogen conversion efficiencies were similar for sucrose (23{\%}) and somewhat lower for molasses (15{\%}) but were much lower for lactate (0.50{\%}) and cellulose (0.075{\%}).",
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Biological hydrogen production measured in batch anaerobic respirometers. / Logan, Bruce Ernest; Oh, Sang Eun; Kim, In S.; Van Ginkel, Steven.

In: Environmental Science and Technology, Vol. 36, No. 11, 01.06.2002, p. 2530-2535.

Research output: Contribution to journalArticle

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