Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability

Cheng Dong, Xiao X. Lei

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The mechanics of leukocyte (white blood cell; WBC) deformation and adhesion to endothelial cells (EC) has been investigated using a novel in vitro side-view flow assay. HL-60 cell rolling adhesion to surface-immobilized P-selectin was used to model the WBC-EC adhesion process. Changes in flow shear stress, cell deformability, or substrate ligand strength resulted in significant changes in the characteristic adhesion binding time, cell-surface contact and cell rolling velocity. A 2-D model indicated that cell-substrate contact area under a high wall shear stress (20dyn/cm2) could be nearly twice of that under a low stress (0.5dyn/cm2) due to shear flow-induced cell deformation. An increase in contact area resulted in more energy dissipation to both adhesion bonds and viscous cytoplasm, whereas the fluid energy that inputs to a cell decreased due to a flattened cell shape. The model also predicted a plateau of WBC rolling velocity as flow shear stresses further increased. Both experimental and computational studies have described how WBC deformation influences the WBC-EC adhesion process in shear flow. Copyright (C) 1999.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalJournal of Biomechanics
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2000

Fingerprint

Biomechanics
Shear flow
Formability
Biomechanical Phenomena
Cell Adhesion
Endothelial cells
Adhesion
Shear stress
Cell adhesion
Endothelial Cells
Leukocytes
Substrates
Assays
Energy dissipation
Mechanics
P-Selectin
Blood
Cell Shape
HL-60 Cells
Ligands

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

Cite this

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title = "Biomechanics of cell rolling: Shear flow, cell-surface adhesion, and cell deformability",
abstract = "The mechanics of leukocyte (white blood cell; WBC) deformation and adhesion to endothelial cells (EC) has been investigated using a novel in vitro side-view flow assay. HL-60 cell rolling adhesion to surface-immobilized P-selectin was used to model the WBC-EC adhesion process. Changes in flow shear stress, cell deformability, or substrate ligand strength resulted in significant changes in the characteristic adhesion binding time, cell-surface contact and cell rolling velocity. A 2-D model indicated that cell-substrate contact area under a high wall shear stress (20dyn/cm2) could be nearly twice of that under a low stress (0.5dyn/cm2) due to shear flow-induced cell deformation. An increase in contact area resulted in more energy dissipation to both adhesion bonds and viscous cytoplasm, whereas the fluid energy that inputs to a cell decreased due to a flattened cell shape. The model also predicted a plateau of WBC rolling velocity as flow shear stresses further increased. Both experimental and computational studies have described how WBC deformation influences the WBC-EC adhesion process in shear flow. Copyright (C) 1999.",
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Biomechanics of cell rolling : Shear flow, cell-surface adhesion, and cell deformability. / Dong, Cheng; Lei, Xiao X.

In: Journal of Biomechanics, Vol. 33, No. 1, 01.01.2000, p. 35-43.

Research output: Contribution to journalArticle

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